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PROTEINS ARE THE MACROMOLECULES COMPOSED OF AMINO ACIDS. EVERY PROTEIN HAS A PARTICULAR SEQUENCE OF THE AMINOACIDS CALLED THE PRIMARY STRUCTURE WHICH FOLDS TO PROVIDE THE PROTEIN A PARTICULAR 3D STRUCTURE. ON THIS 3D STRUCTURE LIES THE EPITOPES OR REGIONS THAT CAN BE IDENTIFIED BY THE ANTIBODIES OR IMMUNOGLOBLINS. TO VISUALIZE A PROTEIN IN A TISSUE ONE NEEDS TO HAVE PRIMARY ANTIBODY PARTICULAR TO THE PROTEIN IN QUESTION AND THE LABELED SECONDARY ANTIBODY SPECIFIC FOR THE PRIMARY ANTIBODY. ONE CAN USE FLUROSCENT MICROSCOPES TO VISUALIZE THE PROTEINS BY TAGGING THEM WITH A FLUROCHROME ON SECONDARY ANTIBODY. GAGANJOT

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Q: How do you observe protein in a tissue?
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