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By adding a specific reagent.
It can help in the purification of substance that are soluble in any solvent and also this helps because every substance has a different retention factor so they will all appear on different levels in the chromatogram this is used in the field of medicine to obtain extremely pure chemicals for the production of drugs. Answered by XCESS
So that you can measure how far it traveled.
Locating agent is a substance/liquid that help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.
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Towards the centre of the chromatogram. The smaller molecules are more mobile and appear towards the outside of the chromatogram.
During the development of a chromatogram, it is better to cover it with a beaker to prevent evaporation, which will affect the movement of the solute and solvent system. This will ensure that the chromatogram will develop properly.
Two ways of identifying amino acids on a chromatogram are paper and gas.
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The chromatogram, or chromatography paper has solvent on it. Each pigment will separate itself from the others due to the relative solubility of that particular pigment.
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By adding a specific reagent.
it is because sweat and other substances especially colored ones may be present there. so it is best not to touch chromatogram papers to aviod contaminating it and getting erroneous results..:) chem.student..:)
Resorcinol is a colouring agent for some chromatography experiments.
Bad things happen. The mobile phase evaporates and thus it's composition changes. The vapor phase above the chromatogram is not saturated or at equilibrium, and this affects the movement of the sample on the solid phase.
It help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.