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Dissolve enough sodium acetate(or other acetate) to reach .5 M(which is .5Mol/L) you use the molecular weight of the acetate and the mass of the acetate to figure out how much to add. Then once dissolved in the appropriate amount of water simply adjust the pH using a strong acid or base until the pH is 6.0.
Wiki User
∙ 15y ago
Wiki User
∙ 14y agoYou need to know the molarity of the acetate first. Then use the following equation where M = molarity and V = volume
MaVa = MbVb
Solve for your unknown (Va)
After the buffer is made, use a pH meter to check the pH. If it is too high, use an acid. If it is too low, use a base.
Wiki User
∙ 14y ago- pHbuffer = 4.5
- acid = acetic acid, amount in mmoles, unknown = A (mmol) = Volume(L)*conc(mmol/L)
- base= acetate, amount in mmoles= 5.0 mmol = 0.100(L)*50(mmol/L)
- pKa=4.77
Calculation:
[ acid / base ] = INVlog[ pKa - pH ] = INVlog[ 4.77 - 4.5 ] = INVlog[0.27] = 100.27 = 1.86 (a/b)
So if base (acetate) is to be 5.0 mmol (present in 100 ml) then acid (acetic) has to be 9.3 mmol. (because a/b has to be 9.3/5.0= 1.86)
Suppose you also have already an 100 mM Acetic acidsolutiom (0.10 M) then you'll need 93 mL to add to the original 100 ml of 50 mM acetate solution.
Wiki User
∙ 11y agoA typical approach to this would be to first create two solutions; one of sodium acetate and one with acetic acid. They preferably need to be of the same concentration which is the desired buffer molarity. Typical buffer molarities would be 25mM to 150mM acetate.
You add a constant pH monitor and put one of the two buffers (start with the one closest to 5.5, so the acetic acid solution) into a beaker and add the other one until pH 5.5 is reached.
That is the most rigorous method that would be for something precise like X-Ray crystallography. Remember to record the volume of each that necessary to reach 5.5 in case you must do it again.
The less rigorous and faster method is to use the sigma aldrich buffer calculator (see Related Links) and then test final pH and adjust with HCl and NaOH. If salt is added this ratio might be a little off.
Word of advice, never trust a buffer recipe to be exact for biochemistry. Always test it with a calibrated pH probe.
Wiki User
∙ 15y agohow acetic acid maintain pH in plant rhizosphere how acetic acid maintain pH in plant rhizosphere
Wiki User
∙ 8y agoMix 357 mL acetic acid 0,1 M with 643 mL sodium acetate 0,1 M.
Wiki User
∙ 15y agono
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Sodium acetate buffer?
can I make sodium acetate buffer 0.2M Ph=5 whit sodium acetate buffer 3m Ph=5 solution?
Why does the pH of an acetate buffer increases after dilution with water?
The pH of water is approximatly 7 (a neutral pH), and the acetate buffer has an acidic pH (less than 7) so when you add distilled water to the buffer the pH will increase.
How do you prepare 100mM ammonium acetate?
To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. Adjust 1 L of this solution to pH 4.5 by adding acetic acid (about 8 ml) and 5 ml of 1 M p-TSA (equivalent to 5 mM p-TSA).
How do you adjust pH of ammonium acetate solution to?
the pH of ammonium acetate buffer can be adjusted with glacial acetic acid (concentrate or diluted )
What is the role of sodium acetate buffer in casein estimation from milk?
pH of sodium acetate buffer is 4.6 and most of the proteins have 4.8 isoelectric pH (pI), so buffer maintains the pI of casein in the casein estimation from milk
Sodium acetate buffer?
can I make sodium acetate buffer 0.2M Ph=5 whit sodium acetate buffer 3m Ph=5 solution?
Why does the pH of an acetate buffer increases after dilution with water?
The pH of water is approximatly 7 (a neutral pH), and the acetate buffer has an acidic pH (less than 7) so when you add distilled water to the buffer the pH will increase.
What is the pH of Triethylammonium acetate buffer?
7
How do you prepare 100mM ammonium acetate?
To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. Adjust 1 L of this solution to pH 4.5 by adding acetic acid (about 8 ml) and 5 ml of 1 M p-TSA (equivalent to 5 mM p-TSA).
How do you adjust pH of ammonium acetate solution to?
the pH of ammonium acetate buffer can be adjusted with glacial acetic acid (concentrate or diluted )
How to prepare phosphate buffer pH 5.8?
preparation of 5.8 ph phosphate buffer
What is the role of sodium acetate buffer in casein estimation from milk?
pH of sodium acetate buffer is 4.6 and most of the proteins have 4.8 isoelectric pH (pI), so buffer maintains the pI of casein in the casein estimation from milk
Why acetate buffer is used while isolating casein from milk?
pH of sodium acetate buffer is 4.6 and most of the proteins have 4.8 isoelectric pH (pI), so buffer maintains the pI of casein in the casein estimation from milk
How do you prepare acetate buffer pH 3.5?
Dissolve 25g of Ammonium acetate in 25ml of water and add 38ml of 7M hydrochloric acid. Adjust the pH of the solution to 3.5 with either 2M hydrochloric acid or 6M ammonia and dilute with water to 100ml
Why pH of sodium acetate is always adjusted with glacial acetic acid only?
in order to form acetate/acetic acid buffer solution!
How do you prepare phosphate buffer of pH 7.5?
See the Related Links for "Columbia.edu: Phosphate buffer automatic calculator" to the bottom for the answer.
When Dr. Farrell was a graduate student he once made up a pH 8.0 sodium acetate buffer. Why would the casual observer to this buffering faux pas come to the conclusion that he?
Not exactly sure what the question is asking, but sodium acetate will not buffer at pH 8. It is an ok buffer in the pH range of maybe 3-5 or so. Acetic acid is the weak acid of this buffer with a pKa near 10^-5. To make a decent buffer at pH 8, one needs a weak base, or a weak acid with a pKa closer to 8.
