Acetocarmine stains chromosomes and sterile pollen must lack them.
it is a genetic stain by which chromosomes can be distinctly seen
In pollen context, acetocarmine is used as a weak stainer for cytoplasm, unlike in chromosome studies where it stains nucleic acids.
Chromosomes are easiest to see when they are condensed(coiled and supercoiled, so that they become short and fat).They are at their most condensed from late prophase through to early telophase, in either mitosis or meiosis.Their visibility also depends on suitable microscopic technique, including the use of an appropriate stain, such as acetocarmine.
Cytogeneticists prepare mitotic cells in samples and stain the chromosomes for visual examinations under the microscope to see if there is any abnormalities in the number of chromosomes and/ or in the structure of the chromosomes.
Acetocarmine stains chromosomes and sterile pollen must lack them.
Cochineal (Dactylopius coccus)
it is a genetic stain by which chromosomes can be distinctly seen
Acetocarmine is a DNA specific stain like feulgen stain, so the super coiled chromosomes during different stages of mitosis present in the Onion root tip cells can be visualized perfectly by treating with this stain.
we use acetocarmine to stain chtomosomes. stained chromosomes distinguish from the other organels and materials, it gives the chromosomes a red color. it enables that, during the cell cyle we can observe the nucleus, duplication of DNA, and movement.
In pollen context, acetocarmine is used as a weak stainer for cytoplasm, unlike in chromosome studies where it stains nucleic acids.
take the root tip (about 1 cm )and put it in dil. HCL solution for ten min. take the tip out and put in acetocarmine stain for 1min. take out the root put it in water bto remove extra stain .
Acetocarmine is acetic acid as solvent with dissolved carmine
0.15g of leishman powder dissolved in 100ml of distilled water
In order to stain your filing cabinet a new color you will have to prepare it first. You will have to strip the old finish off and then sand the wood and prime it so it is ready to accept the new color. If you don't want to do all of the prep work you could look for a primer paint and then an oak color and try painting over it, but it will not look nearly as nice as it will if you strip it, prepare it and stain it.
schiller's iodine solution
Chromosomes are easiest to see when they are condensed(coiled and supercoiled, so that they become short and fat).They are at their most condensed from late prophase through to early telophase, in either mitosis or meiosis.Their visibility also depends on suitable microscopic technique, including the use of an appropriate stain, such as acetocarmine.