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Q: How do you prepare DCPIP solution1?
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How do you prepare DCPIP solution?

1 % DCPIP solution can be prepared by dissolving 1g of the dye in 100cm3 of water


What is DCPIP?

The fullform of DCPIP is 2,6- dichlorophenolindophenol.


Which form of DCPIP reduced or oxidized is higher in energy?

DCPIP oxidized is higher in energy


What are the risks of DCPIP?

hmmm.. Safety gloves should be worn in case of a spillage of DCPIP but it's optional. Spilling DCPIP may cause staining on the skin or the surface you are working at. Risks of DCPIP Are extremely low. Do not consume the DCPIP or purposely inhale the substance in case of any dangers or problems related with doing so.


Is dcpip harmful?

no its not


How does dcpip in the hill reaction affect absorbance?

DCPIP is a blue colour when its oxidized and when it is reduced it turns into a colourless solution. DCPIP replaces NADPH the final electron acceptor in the light dependent reaction. So basically, as the reaction continues, the more DCPIP is reduced and the absorbance decreases.


Are chloroplast necessary for reduction of DCPIP and why?

Chloroplasts release electrons when stimulated by light the DCPIP accepts the electrons and becomes colourless


What is the mass of DCPIP powder needed to form 300 mL of 1percent DCPIP solution and all measurements in grams and millilitres?

3 g


What is a DCPIP indicator for?

It can be used to test for vitamin C


What colour will the DCPIP test give if it is positive?

it will become colourless.


How does DCPIP work as an indicator in the detection of Vitamin C?

Yes it does (I know because i did the test on orange juice) The more Vitamin C something has in it the less drops of that thing (in my case the orange juice) is needed for the DCPIP to go pale yellow. It is an awesome test! I would try it ( only if you have DCPIP and lots of different types of orange juice! Of course!)


Why is the color change proof of the presence of mitochondria?

To find out if we have mitochondria, we add DCPIP. The tube that has the most mitochondria will change color most from Blue to Clear. This is because mitochondria has an enzyme called Succinic Dehydrogenase. This enzyme can be picked up by removing (2) Hydrogen atoms and the DCPIP picks those Hydrogen atoms up, becoming DCPIP2. Note that DCPIP is DARK BLUE, and DCPIP2 is CLEAR. So, when the hydrogen is picked up and DCPIP becomes DCPIP2, the color goes clearer, and so there is mitochondria present. Info generated from Lab done in class (Mr. Wilson, Bio 181) Mohamad Ali Zeitoun