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GLYCINE- NAOH BUFFER; PH 8.6-10.6, PKA = 9.78

Stock solutions:

0.2 M glycine

0.2 NaOH

Combine 25 ml glycine stock solution with x ml 0.2 M NaOH and dilute with DI to make a 100 ml solution (Pearse, 1980).

{| ! width="133" height="15" align="middle" | 0.2 M NaOH|} {| ! width="114" height="15" align="middle" | pH |} {| ! width="133" height="15" align="middle" | 2.0 |} 8.6 {| ! width="133" height="15" align="middle" | 3.0 |} 8.8 {| ! width="133" height="15" align="middle" | 4.4 |} 9.0 {| ! width="133" height="15" align="middle" | 6.0 |} 9.2 {| ! width="133" height="15" align="middle" | 8.4 |} 9.4 {| ! width="133" height="15" align="middle" | 11.2 |} 9.6 {| ! width="133" height="15" align="middle" | 13.6 |} 9.8 {| ! width="133" height="15" align="middle" | 19.3 |} 10.4 {| ! width="133" height="15" align="middle" | 22.75 |} 10.6

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15y ago
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1d ago

To prepare a glycine-sodium hydroxide buffer at pH 9.2, start by dissolving the desired amount of glycine in water. Adjust the pH to 9.2 using sodium hydroxide solution. Finally, dilute the solution to a final concentration of 1M by adding water. Stir well and check the pH again if needed.

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Q: How do you prepare glycine sodium hydroxide buffer pH 9.2 1M?
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