you can prepare 20nM sodium phosphate buffer pH7.0.
calculate mass of enzyme and dilute in volume buffer to reach concentration you want.
good luck
A solute and solvent make up a solution. The solute is the substance that is dissolved in the solvent to form the solution. The solvent is the substance in which the solute is dissolved.
To make a potassium thiocyanate solution, first dissolve potassium thiocyanate powder in distilled water. Stir the mixture until the powder is completely dissolved. The resulting solution can be used for various chemical reactions or experiments.
To make a 1.00M NaCl solution from a 2.00M solution, you can dilute the 2.00M solution by adding an equal volume of solvent (like water). For example, mix 1 cm^3 of the 2.00M solution with 1 cm^3 of water to create a 1.00M solution.
To make a percent sucrose solution, dissolve a specific weight of sucrose in a specific volume of water. For example, to make a 10% sucrose solution, dissolve 10 grams of sucrose in 90 mL of water. The formula to calculate the amount of sucrose needed is: (percent sucrose/100) x volume of solution = weight of sucrose (in grams).
To make a 20 vol peroxide solution from a 40 vol peroxide solution, you need to dilute the 40 vol solution by mixing it with an equal amount of water. This will effectively reduce the concentration of the peroxide solution by half, resulting in a 20 vol solution.
No, E. coli is urease-negative
Urea which is protein substrate
The enzyme responsible for metabolizing urea is urease. Urease breaks down urea into ammonia and carbon dioxide.
most definitely. it is a strong positive urease producer.
Urease breaks down the compound urea into ammonia and carbon dioxide.
Urease positive means, when there is trace of urease in blood/urine of some subject. Urease is an enzyme that changes urea into ammonium carbonate and (occurring in bacteria, fungi, etc.)
Can you relate urease production to a diaper rash in infants
No. Streptococcus species are not urease positive.
Proteus urease is considered a positive organisim. This occurs when enterics hydrolyze urea and cause a rapid deterioation. This term is also referred to as rapid urease positive organisms.
Because M.tuberculosis produces urease. thus indicating a person has the bacteria can cause tuberculosis.
No, urease and urea are not the same thing. Urease is an enzyme that catalyzes the hydrolysis of urea into ammonia and carbon dioxide, while urea is a waste product produced in the liver as a result of protein metabolism.
yes