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How do you prepare streptozotocin solution for the induction of diabetes?
Wiki User
∙ 11y ago
Version: 1
Replaced by version: N/A
Edited by: The University of Michigan Medical Center , Frank Brosius
Summary
Reagents and Materials
Protocol
Reagent Preparation
Na-Citrate Buffer
Streptozotocin (STZ)
STZ-Na-Citrate Solution
Summary: This protocol is used by AMDCC members to induce Diabetes in a number of the animal models developed by the consortium. This induction protocol is used to induce a diabetes similar to a type I diabetic.
Reagents and Materials: Reagent/Material
Quantity Required
Vendor
Stock Number
STZ-Na-Citrate Solution
See below
TB or insulin syringes w/ needle attached (26-28 ½ gauge)
As many as necessary for the number of mice
Isoflurane drop jar
Isoflurane
Wiki User
∙ 11y ago
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