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Capsules may be revealed by methods such as Maneval's method. This method utilizes negative staining, where the background is stained revealing an unstained structure: the bacterial capsule.
Preperation of slides and Leifson flagella stain?
Because violet dye is the least expensive.
The preparation of insoluble salt can be done by PRECIPITATION METHOD
The hot method uses heat to force the primary stain (carbol fuchsin) into the cell wall, while the cold method uses a detergent to cause the stain to penetrate the wall.
H & E staining is good as a primary staining method alone. The selection of a relevant staining method depends on the type of sample you are planning to visualize. Re post with said detail to help you pick the right stain.
The Gram staining method, named after the Danish bacteriologist who originally devised it, started in 1882.
Capsules may be revealed by methods such as Maneval's method. This method utilizes negative staining, where the background is stained revealing an unstained structure: the bacterial capsule.
I sand it with a final sanding of 400 grit paper, then scrub it with 0000 steel wool, wipe it of carefully then give it first staining with a rag dipped in the stain.
Preperation of slides and Leifson flagella stain?
Because violet dye is the least expensive.
to prepare a response sheet
schiller's iodine solution
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
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