example of gel is agarose gel,
the thickness of the gel varies as you change the conc. of agarose from 2 to 3 percent. the conc. of loading of samples that gel can handle varies.
The agarose gel acts as a matrix that slows down the dna segments as they move to the opposite charged end of the gel. A larger segment will have a tougher time moving through the gel, while a smaller segment will move faster because it is easier to move it through the gel.
increasing the agarose concentration will enable the separation of smaller fragments of DNA. the structure of the gel (agarose) consists of crosslinks, therefore the higher the concentration of agarose the more crosslinks there will be and smaller size "holes" for the DNA to travel through (also the other way around, with less concentrated agarose)
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
example of gel is agarose gel,
Check the answer for How do you make an electrophoresis gel?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
Agarose gel electrophoresis is suitable for ALL DNA.
Stuff
Agarose is a linear polysaccharide used for gel mediums. Tm (melting temp) is about 85 C.
The larger fragements will not be very accurate because they cannot resolve in high consentrations of the agarose in the gel. The percent of agarose in the gel affects the ability to resolve larger fragements of DNA
the thickness of the gel varies as you change the conc. of agarose from 2 to 3 percent. the conc. of loading of samples that gel can handle varies.
The agarose gel acts as a matrix that slows down the dna segments as they move to the opposite charged end of the gel. A larger segment will have a tougher time moving through the gel, while a smaller segment will move faster because it is easier to move it through the gel.
increasing the agarose concentration will enable the separation of smaller fragments of DNA. the structure of the gel (agarose) consists of crosslinks, therefore the higher the concentration of agarose the more crosslinks there will be and smaller size "holes" for the DNA to travel through (also the other way around, with less concentrated agarose)
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
No, the agarose gel is just a polysaccharide.