You have performed a transformation experiment using a mutant strain that is arg-- trp+ gal-- his--. A culture of this mutant strain was mixed with a DNA from another mutant strain that is arg+ trp-- gal+ his+.
How will you select for the following recombinants?
a) arg+ trp+ gal-- his--
b) arg+ trp-- gal+ his+
c) arg-- tryp-- gal+ his--
d) arg-- trp+ gal-- his+
You use a HAT medium
I'm not quite sure what you mean by your question, but if you are asking about what organisms contain the DNA of another organism, the human body could count as one. The mitochondria within our cells have a DNA that is different from our own DNA strands, which caused many scientists to believe that they might have once been another organism all-together. They replicate using their own processes, too. Hope that helps!
Recombinant- is the DNA fragment of interest Nonrecombinant- the undesired foreign DNA
When DNA contains parts from two or more organisms it is recombined. Recombinant DNA is often used in genetic engineering. A natural process of DNA recombination is called sexual reproduction.
eg for organisms which carry recombinant DNA : Ecoli
Recombinant DNA is a new form of DNA because it is created via introduction of the relevant DNA into the existing organismal DNA.
Chimeras. In genetic engineering, molecules of combined DNA are known as chimeras because they are produced by combining DNA from different species. Combined DNA is also known as recombinant DNA, since DNA from 2 sources has been recombined to produce it.
1.Cleaving DNA The source chromosomes is cut into fragments of DNA.2.Producing recombinant DNA The DNA fragments containing the desired gene are inserted into viral or bacterial DNA. The recombinant DNA is then allowed to infect the target cells.3.Cloning cells infected cells are allowed to reproduce.Growing a large number of identical cells from one cell is known as cloning.4.Screening target cells Targets cells that have received the particular gene of interest are isolated.
I'm not quite sure what you mean by your question, but if you are asking about what organisms contain the DNA of another organism, the human body could count as one. The mitochondria within our cells have a DNA that is different from our own DNA strands, which caused many scientists to believe that they might have once been another organism all-together. They replicate using their own processes, too. Hope that helps!
In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.
The word you're looking for may be "recombinant".
pcDNA is DNA vector used to clone recombinant DNA sequences for the expression of proteins of interest in mammalian cells.
In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.
Recombinant- is the DNA fragment of interest Nonrecombinant- the undesired foreign DNA
When DNA contains parts from two or more organisms it is recombined. Recombinant DNA is often used in genetic engineering. A natural process of DNA recombination is called sexual reproduction.
eg for organisms which carry recombinant DNA : Ecoli
Recombinant DNA is a new form of DNA because it is created via introduction of the relevant DNA into the existing organismal DNA.
There are steps you must follow in the making of recombinant DNA such as use crosses to identify donor, clone gene in bacterium, characterize the gene, modify the gene, and reintroduce the gene into donor cells.