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DNA and RNA is stained by making the gel with 1 or 2 drops of ethidium bromide, which is a dye specific for nucleic acids and can be visualized under UV light.
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Are electrophoresis gels hazardous?
No, the agarose gel is just a polysaccharide.
What is lambda DNA?
sheesh are you in the wrong place, it is DNA from a virus that can infect E. coli and is often used as molecular weight standards on agarose gels.
Can we use the UV light in laminar air flow to see DNA bands in agarose gels stained with Ethidium bromide?
One cannot use the UV light installed in a laminar air flow hood to visualize DNA in an agarose gel. You will have to use an instrument called a UV transillumunator, which illuminates the gel from below to see the stained DNA.
Minimum DNA on agarose to visualize?
you need at least 20ng to visualize it on an agarose gel along with EtBr or GR safe
Why you have the range of concentration of agarose in gel electrophoresis?
increasing the agarose concentration will enable the separation of smaller fragments of DNA. the structure of the gel (agarose) consists of crosslinks, therefore the higher the concentration of agarose the more crosslinks there will be and smaller size "holes" for the DNA to travel through (also the other way around, with less concentrated agarose)
What is an agarose?
An agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar and used to make gels.
Are electrophoresis gels hazardous?
No, the agarose gel is just a polysaccharide.
Factors that affect the rate of DNA migration in Agarose gels electrophoresis.?
size of the DNA
What are DNA gels?
DNA gels is a term that usually refers to agarose gels, made with TAE (Tris, Acetate, EDTA) or TBE (Tris, Borate, EDTA) buffer. They are the simplest to make and don't contain toxic compounds (unless EtBr is added to the gel).
What is differences between agar and agarose?
Agarose is made from agarose, a polysaccharide from see weeds. Polyacrylamide is made from the synthetic polymerization of acrylamide, which in its monomeric form is a neurotoxin. Based on these structural differences, it could be said that agarose gels have larger 'pores' than polyacrylamide gels meaning that large particles can move more easily in agarose gels since the agarose polymers are larger and pack less densely then an equivalent amount of polyacrylamide. Therefore, agarose is generally used for the electrophoresis of large molecules such as DNA and RNA or speedy separation (low resolution) of small molecules such as proteins. Polyacrylamide is used for the high resolution electrophoresis of small molecules such as proteins.
What is lambda DNA?
sheesh are you in the wrong place, it is DNA from a virus that can infect E. coli and is often used as molecular weight standards on agarose gels.
Can we use the UV light in laminar air flow to see DNA bands in agarose gels stained with Ethidium bromide?
One cannot use the UV light installed in a laminar air flow hood to visualize DNA in an agarose gel. You will have to use an instrument called a UV transillumunator, which illuminates the gel from below to see the stained DNA.
What is role of agarose of electrophoresis?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
What is agarose and what is the TM value of agarose?
Agarose is a linear polysaccharide used for gel mediums. Tm (melting temp) is about 85 C.
Minimum DNA on agarose to visualize?
you need at least 20ng to visualize it on an agarose gel along with EtBr or GR safe
Preparation of 1 percent agarose gel or How to prepare 1 percent agarose gel?
Check the answer for How do you make an electrophoresis gel?
Is agarose gel electrophoresis suitable for only gram negative plasmid DNA?
Agarose gel electrophoresis is suitable for ALL DNA.
