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What is the role of DEPC in RNA isolation?
DEPC (diethylpyrocarbonate) is often used in RNA isolation to inactivate RNases, which are enzymes that can degrade RNA. DEPC is commonly added to water used in RNA isolation procedures to ensure that RNases are deactivated, thus helping to preserve the integrity of the RNA being isolated.
How does DEPC inactivate RNAases exactly?
DEPC (diethyl pyrocarbonate) inactivates RNAases by reacting with histidine residues in the active site of the enzyme. This chemical modification disrupts the structure and function of the RNAase, rendering it inactive. DEPC treatment is commonly used to eliminate RNAases in solutions and labware for RNA-related experiments.
How DEPC water is prepared?
DEPC water is prepared by adding diethyl pyrocarbonate (DEPC) to distilled water in a final concentration of 0.1%. The solution is then left to stand overnight at room temperature to allow DEPC to hydrolyze and become inert. The DEPC-treated water is then autoclaved to remove any remaining traces of the chemical.
While doing RNA emsa is it necessary to use depc?
in any procedure where RNA is used, DEPC is required. This component ensures the integrity of RNA and prevents unnecessary RNA degradation during the course of the experiment
Can 20x SSC buffer be DEPC-treated?
yes it can be
What is the solubility of a yeast RNA in a cold water?
Yeast RNA is generally soluble in cold water, as it is a hydrophilic molecule. However, factors such as the presence of salts, pH, and RNA size can influence its solubility. If you encounter solubility issues, you can try adjusting these factors or using different solvents such as DEPC-treated water or TE buffer.
What is the purpose of RNA extraction?
RNAse destroys the RNA and hence RNAse contamination is a problem in RNA extraction as it breaks down RNA. RNAse enzyme is removed by using RNAse inhibitor or precautions like wearing of gloves, autoclaving tips , using RNAse free water/DEPC treated water is done while performing RTPCR
What kind of gun licence do I need to tail dock dogs?
In the UK only Veterinarians are permitted to dock puppies/dogs' tails. It is illegal for a breeder/lay person to do it.Scotland - tail docking except for therapeutic reasons has been banned in Scotland as from 30th April, 2007. They cannot be taken across borders to be docked.N. IRELAND - No change as yet from previous legislation where only Veterinary Surgeons may dock.REGULATIONS:-ENGLAND - The regulations for truly WORKING dog exemptions as from 6th April 2007 (although some may be found not to be suitable for work later) are Terriers and their crossbreeds, Spaniels and their crossbreeds and HPRs. This does not apply to dogs bred as pets.Anyone who is cruel to an animal, or does not provide for its welfare needs, may be banned from owning animals, fined up to £20,000 and/or sent to prison.http://www.opsi.gov.uk/acts/acts2006/ukpga_20060045_en_1http://www.opsi.gov.uk/acts/acts2006/en/ukpgaen_20060045_en.pdfhttp://www.opsi.gov.uk/acts/acts2006/en/ukpgaen_20060045_en_1.htm - endNB 8) A person commits an offence if- (a) he owns a subsection (3) dog, and (b) fails to take reasonable steps to secure that, before the dog is 3 months old, it is identified as a subsection (3) dog in accordance with regulations made by the appropriate national authority.Explanatory note:-31. Subsection (8) requires a person who owns a dog which was legitimately docked by a Vet to ensure that the dog is identified as having been legally docked. The owner will commit an offence if he does not take reasonable steps to ensure that his docked dog is so identifiedSubsection (13)(b) allows the appropriate national authority to make regulations about the method of identification required, e.g. micro-chipping .WALES - The list of truly working dog breeds exempted and as amended in the Welsh Plenary as from 28th March 2007 (although some may be found not to be suitable for work later) is now as follows:Spaniels: of only the following breeds will be eligible English Springer Spaniel; Welsh Springer Spaniel; Cocker Spaniel, but not combinations of breeds andTerriers of only the following breeds will be eligible Jack Russell Terrier, Cairn Terrier (normally undocked!) Lakeland Terrier, Norfolk Terrier - but not combinations of breeds. This does not apply to dogs bred as pets.The HPR breeds remain as originally drafted. Bracco Italiano; Brittany; German Long-haired Pointer; German Wire-haired Pointer; Hungarian Vizsla; Hungarian Wire-Haried Vizsla; Italian Spinone; Spanish Water Dog; Weimaraner; Korthals Griffon; Slovakian Rough-Haired Pointer; Large Munsterlander; Small Munsterlander.This does not apply to dogs bred as pets.http://new.wales.gov.uk/topics/environmentcountryside/ahw/animal_welfare/Companiondomesticanimalwelfare/taildockingregulations/?lang=enCertificate requiredhttp://new.wales.gov.uk/depc/1382175/1519627/DOC_1_CERTIFICATE_ENGLISH_11.pdf?lang=enPuppies presented for docking to the Vet as truly WORKING dogs can only be docked at the discretion of the Vet.A person commits an offence if he shows a dog at an event to which members of the public are admitted on payment of a fee (England and Wales), also see these relevant DEFRA links:-http://www.opsi.gov.uk/si/si2007/20071120.htmhttp://www.defra.gov.uk/animalh/welfare/act/index.htmhttp://www.defra.gov.uk/animalh/welfare/act/docking.htm
