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What does the acronym EMSA stand for?
The acronym EMSA stands for quite a variety of words. The two most common combination of words the acronym stands for are, Electrophoretic Mobility Shift Assay and European Medical Students Association.
How does EMSA electromobility shift assay measure whether the protein bends DNA?
EMSA does not measure if protein bends to DNA. It does measure what proteins bind to a specific region of DNA (usually a promoter region). You can use a supershift to determine exactly what protein is binding to the specific DNA region.
What is Electromobility shift assay?
One method for detecting DNA-protein interaction. EMSA rely on the ability of proteins to bind radiolabels DNA fragments in vitro. Once binding occurs, DNA-protein complexes can be separated from unbound DNA fragments by nondenaturing polyacrylamide gel electroforesis. In general the larger the protein or protein complex bound to the DNA, the greater the extend of retardation of mobility of the radiolabeled DNA fragment. The resulting protein-DNA complexes are visualized by autoradiography.Methods in muscle biology, Charles P. Emerson, H. Lee Sweeney.
What are three techniques used to determine the specific DNA binding site of a DNA-binding protein?
Three techniques used to determine the specific DNA binding site of a DNA-binding protein are electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation (ChIP) assay, and DNase footprinting assay. EMSA involves the visualization of DNA-protein complexes on a gel, ChIP assay identifies DNA fragments bound by the protein in living cells, and DNase footprinting identifies protected regions of DNA from enzyme digestion.
How many people use Non Emergency Medical Transportation in California?
That's a difficult question to answer as California EMSA does not publish this data (http://www.emsa.ca.gov/data_inf/data_inf.asp). There are some national statistics available (http://www.startingabiz.com/starting-a-medical-transportation-business), but none that are state-specific that I could readily find. Good luck!
