What is Electromobility shift assay?

Answer 1

One method for detecting DNA-protein interaction. EMSA rely on the ability of proteins to bind radiolabels DNA fragments in vitro. Once binding occurs, DNA-protein complexes can be separated from unbound DNA fragments by nondenaturing polyacrylamide gel electroforesis. In general the larger the protein or protein complex bound to the DNA, the greater the extend of retardation of mobility of the radiolabeled DNA fragment. The resulting protein-DNA complexes are visualized by autoradiography.

Methods in muscle Biology, Charles P. Emerson, H. Lee Sweeney.

Answer 2

The Electromobility Shift Assay (EMSA) is a biochemical technique used to detect protein-DNA interactions. It involves separating DNA-protein complexes from free DNA through gel electrophoresis, allowing visualization of the binding events. EMSA is commonly used in molecular biology and biochemistry to study transcription factor binding, DNA-protein interactions, and related regulatory mechanisms.