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How does EMSA electromobility shift assay measure whether the protein bends DNA?
Wiki User
∙ 16y ago
EMSA does not measure if protein bends to DNA. It does measure what proteins bind to a specific region of DNA (usually a promoter region). You can use a supershift to determine exactly what protein is binding to the specific DNA region.
Wiki User
∙ 16y ago
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If the mitochondrion is fundmental to the mtt assay which organelle plays a key role in the neutral red viability assay and why?
lysosome
What amino acid residues are detected when the spectrophotometric assay is used to quantify proteins?
In Lowry spectrophotometric assay, tryosine, tryptophan, cysteine, histidine, and asparagine.
What is the intact PTH assay used for?
The assay for intact PTH and the N-terminal fragment, which are both measured at the same time, is more accurate in detecting sudden changes in the PTH level. For this reason, the N-terminal PTH assay is used to monitor a patient's response to therapy.
What is the LAL assay?
The LAL test is a method for the detection of bacterial endotoxins.
Full form of WIDAL?
wide investigation diagnosis assay laboratory
What is protein assay?
Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
What is Electromobility shift assay?
One method for detecting DNA-protein interaction. EMSA rely on the ability of proteins to bind radiolabels DNA fragments in vitro. Once binding occurs, DNA-protein complexes can be separated from unbound DNA fragments by nondenaturing polyacrylamide gel electroforesis. In general the larger the protein or protein complex bound to the DNA, the greater the extend of retardation of mobility of the radiolabeled DNA fragment. The resulting protein-DNA complexes are visualized by autoradiography.Methods in muscle biology, Charles P. Emerson, H. Lee Sweeney.
What is a p24 antigen capture assay?
Found in the viral core of HIV, p24 is a protein that can be measured by the ELISA technique. Doctors can use p24 assays to measure the antiviral activity of the patient's medications. In addition, the p24 assay is sometimes useful.
What is a bandshift assay?
A bandshift assay is a type of assay using gel electrophoresis, in which the mobility of a DNA or RNA probe alone is compared to its mobility in combination with a particular protein.
Is the Bradford dye method of protein assay considered destructive or non destructive?
non-destructive
Merits and demerits of Bradford assay?
Bradford protein assay is a very quick way of testing for proteins in solutions. However, presence of detergents in the solution being investigated renders this test inapplicable.
What does MTT represent in MTT assay?
The MTT in MTT assay represents Methylthiazol TetraZolium. It is part of the field of biochemistry and used to measure enzymes that reduce the tetrazolium dye.
What is azocasein?
Azocasein is a chemically modified protein that was designed as a substrate for quantative assay of proteolytic enzyme activity. Hope that helped!!
What is a marker protien?
A protein marker is just that - a marker for specific proteins. This usually deals with running an experiment (assay) to determine the presence, absence, and with some markers, abundance of a specific protein
What is an assay?
An assaying is a trial by assay, thorough examination and determination.
What is the definition of sensitivity and specificity in a dignostic analysis?
Sensitivity in diagnostic laboratory testing represents the smallest amount of substance in a sample that can be accurately measured by an assay. Specificity in a diagnostic laboratory refers to the ability of an assay to measure one particular organism or substance.
How do you verify gold bar assay certificate?
You can't verify assay certificates.
