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Factors that affect the rate of enzyme activity include temperature, pH, substrate concentration, and enzyme concentration. Temperature and pH can alter the shape of the enzyme, affecting its ability to bind to the substrate. Changes in substrate and enzyme concentration can affect the frequency of enzyme-substrate collisions, which impacts the rate of reaction.
enzyme-substrate complex
in an enzyme-substrate complex, the enzyme acts on the substrate .
Adding another substrate can either increase or decrease the rate at which an enzyme works. If the additional substrate competes with the original substrate for the active site, it can slow down the enzyme activity (competitive inhibition). On the other hand, if the additional substrate binds to a different site on the enzyme and enhances its activity, it can speed up the enzyme reaction.
When an enzyme and substrate come together, it is called the enzyme-substrate complex. This complex is a temporary intermediate state in which the enzyme binds to the substrate to catalyze a chemical reaction.
Concentration of substrate can affect enzyme activity by impacting the rate of enzyme-substrate complex formation. At low substrate concentrations, enzyme activity may be limited by the availability of substrate molecules. However, at high substrate concentrations, enzyme activity may become saturated as all enzyme active sites are occupied.
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3 factors that affect the speed of an enzyme catalysed reaction are: .Temperature .Enzyme Concentartion .Substrate concentration
The substrates are converted into products, which are released.
The size of the enzyme's active site would not contribute significantly to substrate specificity. Substrate specificity is typically determined by the shape, charge, and chemical properties of the active site that can properly bind to the substrate.
A substrate is the substance acted upon by an enzyme. The enzyme substrate complex is when an enzyme molecule combines with its substrates.