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When you are doing your gram stain, you are looking for gram negative and gram positive cells by how they take on the color of the stains that you have applied. The cells would be either pink or purple which would indicate the negative or positive. The age of the cell does not matter for the sake of gram staining. You are looking for negative or positive. The results would lead you to choosing what medication (antibiotics) to use. Some antibiotics work only on gram negative bacteria and some on gram positive.
Wrights staining is where you are looking for the age of the cells for a CBC differential. By identifying the 1. type of cell and 2. age of the cell, you can indicate what is termed as a shift to the left or right. As a cell ages it turns from a one segment (youth) to a hyper- segmented (old) cells. The cell's nucleus will start out as a band and turn into a horseshoe shaped one with lumps. As a person starts to fight off an infection you will see many hyper-segmented (old) WBC's. However, if the person has been fighting off the infection for a long time then you will see more one segmented youth cells because the infection has taken all the older cells. I refer to as like a war. When the war starts out the older people fight. After all the old are gone then you have no choice but to use the younger and younger people until all is gone. That is a shift to the left which is a poor prognosis. A shift to the right is indicating recovery.
What else can I help you with?
What is the gram reaction of s epidermidis?
Staphylococcus epidermidis is a Gram-positive bacterium, meaning it retains the crystal violet stain in the Gram staining procedure. This results in a purple color under the microscope.
Is gram staining related to flagella?
No, gram staining and flagella are not directly related. Gram staining is a technique used to classify bacteria based on cell wall characteristics, while flagella are thread-like appendages that help bacteria move. Flagella presence or absence does not affect the results of a gram stain.
Why can treponema denticola be seen with negative staining?
Because it is a Gram-negative bacterium and can be stained with Gram-negative stain.
What are the acid-fast stain results for Bacillus subtilis?
Bacillus subtilis is a Gram-positive bacterium and does not typically show acid-fast staining results. This means that it does not retain the stain when subjected to the acid-fast staining procedure commonly used to detect mycobacteria.
Is anthrax gram positive or negative?
Anthrax is a gram-positive bacterium, meaning it retains the crystal violet stain in the Gram staining procedure.
Why can tooth and gum bacterium be seen with negative staining?
Not all oral bacteria are gram negative, but those that are there can be seen with Gram stain.
Factor that would give False results in gram staining?
Several factors can lead to false results in Gram staining, including improper sample handling or storage, which may cause bacterial cell degradation. Inadequate fixation of the sample can result in the loss of bacteria during staining. Additionally, over-decolorization or under-decolorization can misclassify Gram-positive bacteria as Gram-negative or vice versa. Lastly, using old or expired reagents can also affect the staining outcome.
What is the name of the paper used to dry slides after gram staining?
Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
What is the color of staphylococcus aureus after gram staining?
Post ID Special:Contributionssaid: "blue-violet.....because it is a gram-negative bacteria"Wrong...the color is correct, but S. aureus is gram-POSITIVE.
What is the gram stain results for an L. lactus bacteria?
Lactococcus Lactus is a gram positive bacteria and therefore retains the darker staining and therefore shows on a gram stain as dark blue/violet colour. This is because the thick peptidoglycan cell wall retains the primary crystal violet stain.
Is b magaterium gram negative?
Bacillus megaterium is not gram-negative; it is a gram-positive bacterium. This classification is based on its thick peptidoglycan cell wall, which retains the crystal violet stain used in the Gram staining procedure. As a result, B. megaterium appears purple under a microscope after staining.
Why is gram staining important?
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
