0
How does ethidium bromide interact with double stranded DNA Does it increase or remove supercoiling?
Wiki User
∙ 11y ago
when ethidium ion intercalates between two dna base pairs in a circular dna it causes the dna to unwind by 26 degrees, thereby decreasing twist and increasing writhe.
in a circular dna which is negatively supercoiled, if ethidium is added it will become relaxed and if more ethidium is added dna becomes positively supercoiled
Wiki User
∙ 11y ago
Add your answer:
Earn +20 pts
Why dont you use ethidium bromide staining for non denaturing PAGE gels?
Because PAGE gels are used to look a protein and ethidium bromide only stains double stranded nucleic acids.
How DNA recoiling into helix?
DNA has the property of two types of coiling:- neative supercoiling and positive supercoiling. It means that it uncoil from one side and at the same time recoil from the next side. the uncoiling is subjected by helicases enzyme and topoisomerases and the ssbp's (single stranded binding proteins let the DNA to stabilize and lets replication to start. while at the same time the DNA recoils from another side, it does so bcoz there is no ssbp's to stabilize DNA so that it doesnt recoil, therefore it recoils.
Why are parts of telomerase RNA double stranded?
Parts of telomerase RNA are double stranded because they form a functional structure called the pseudoknot. The pseudoknot helps stabilize the telomerase RNA and allows it to interact with other components of the telomerase enzyme to carry out its function in telomere maintenance and chromosome stability.
When was Stranded in the Jungle created?
Stranded in the Jungle was created in 1956.
How do you strip insulation off stranded wire?
You would use a set of wire strippers made for stranded wire and proper size. If the strippers are made for stranded and solid make sure you use the size identifications for stranded not solid!
What does ethidium bromide stain in a cell?
Ethidium bromide is a chemical that is able to intercalate (fit between the bases) between the bases of double stranded DNA strands
Why dont you use ethidium bromide staining for non denaturing PAGE gels?
Because PAGE gels are used to look a protein and ethidium bromide only stains double stranded nucleic acids.
Will ethidium bromide stain double stranded DNA or single stranded DNA?
both, though DNA will preferentially form doublestranded DNA at room temperature. it can be quite hard to get single stranded DNA other than by heating your sample up to 95 degrees Celcius.
Why ethidium bromide is carcinogenic?
The purpose of ethidium bromide is as an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it will fluoresce with an orange color, intensifying almost 20-fold after binding to DNA. Hence it is useful in visualizing DNA. Unfortunately, ethidium bromide does not distinguish between what DNA it binds to, whether it be ours or our sample's. Hence ethidium bromide is a mutagen, suspected carcinogen and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract. Ethidium bromide acts as a mutagen because it intercalates into double stranded DNA, thereby deforming the molecule. This is believed to block or trip biological processes occurring on DNA, like DNA replication and transcription.
How DNA recoiling into helix?
DNA has the property of two types of coiling:- neative supercoiling and positive supercoiling. It means that it uncoil from one side and at the same time recoil from the next side. the uncoiling is subjected by helicases enzyme and topoisomerases and the ssbp's (single stranded binding proteins let the DNA to stabilize and lets replication to start. while at the same time the DNA recoils from another side, it does so bcoz there is no ssbp's to stabilize DNA so that it doesnt recoil, therefore it recoils.
Why are parts of telomerase RNA double stranded?
Parts of telomerase RNA are double stranded because they form a functional structure called the pseudoknot. The pseudoknot helps stabilize the telomerase RNA and allows it to interact with other components of the telomerase enzyme to carry out its function in telomere maintenance and chromosome stability.
What do you do in Club Penguin when you are stranded?
stranded where? stranded where? stranded where?
Difference between solid and stranded conductors?
In a solid conductor there will be only one conductor ,but in stranded conductors there are several conductors twisted together to make a whole conductor ,this is mainly done to increase flexibility of the Conductor .
Is trna double stranded or single stranded?
tRNA, or transfer RNA, is a single stranded molecule. The only double stranded RNA is dsRNA, or double stranded RNA. They are typically found in viruses.
To be stranded?
to be stranded is to have no familiar place to go
What might happen if someone is stranded?
it depends on where they are stranded, if they are stranded in a place with no food they might possibly die.
What is dsDNA?
ssDNA stands for single stranded DNA and dsDNA stands for double stranded DNA. ssDNA stands for single stranded DNA and dsDNA stands for double stranded DNA.
