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How does restriction fragment length polymorphism work?

Updated: 9/17/2019
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Q: How does restriction fragment length polymorphism work?
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Do you work? is a complete sentence; it is not a sentence fragment.


What cuts DNA into fragments?

cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.


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How do restriction work?

Restriction enzymes are used for genetic engineering. they expose the base sequence of a DNA fragment. The enzymes cut the phosphate backbones of DNA molecules at specific base sequences called recognition sites. Strands of DNA that have been cut with restriction enzymes sometimes have single-stranded tails that readily realign with tails from certain other DNA fragments. This technology allows removing a specific gene from one organism and splicing it into another. Restriction enzymes originally developed in bacteria as a defense against viruses, who inject DNA in bacteria which takes over the cell. The bacteria's restriction enzymes cut up viral DNA before it can take over the cell.


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What does restriction endonuclease do?

They cut DNA at specific sequences. Restriction endonucleases work by cutting DNA at specific sequences. The places that are cut are known as restriction sites.


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What does the restriction allow?


What determines how DNA will be cut by a restriction enzyme?

Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut


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A restriction of the number of hours 16-year-olds can work. (APEX Class ;)