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E. Coli is typically used as the host of recombinant DNA cloning.
The process is as follows (simple version):
DNA sample is cut use restriction sites, and a primer is loaded
The cut DNA is place in a vector, example is a plasmid
The plasmid is inserted into the host, example is E.Coli
The E.Coli produces the DNA
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∙ 12y ago
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How did Boyer and Cohen performe their recombinant DNA experiment?
Herbert Boyer and Stanley Cohen created the first recombinant DNA organism using recombinant DNA technology, or gene splicing, which allows the manipulation of DNA. They showed that the gene for a frog ribosomal RNA could be transferred and expressed in bacterial cells. Boyer and Cohen removed plasmids, small rings of DNA located in a cell's cytoplasm, from a cell. Using restriction enzymes, they cut the DNA at precise positions and then recombined the DNA strands in their own way using DNA ligase enzyme. They then inserted the altered DNA into E. coli bacteria. The bacterial cells could be made to produce specific proteins using gene splicing. This technology was a major breakthrough for genetic engineering. Their experiments dramatically demonstrated the potential impact of DNA recombinant engineering on medicine and pharmacology, industry and agriculture.
What is a circular DNA strand used in recombinant DNA?
It is called a plasmid. Plasmids are extrachromosomal self-replicating circular forms of DNA present in most bacteria, such as E. Coli, containing genes related to catabolism and metabolic activity,and allowing the carrier bacterium to survive and reproduce in conditions present within other species and environments.
What is recombination DNA?
== == DNA fragments cannot function all by themselves. They must become part of the genetic material of living cells before the gene they contain can be activated. In the second step of genetic engineering, DNA fragments are made into part of the recipient cell's genetic material. This is done by combining DNA fragments with DNA from the recipient cell. For example, DNA fragments may be combined with bacterial DNA so that they can later be inserted into a bacterial cell. Bacteria often contain small circular DNA molecules known as plasmids in addition to their chromosomes. These plasmids can be removed from bacterial cells and cut with the same restriction enzyme used to produce the DNA fragments. The cuts made by the restriction enzyme produce matching "sticky ends" on the DNA fragments and the cut plasmids. These sticky ends are the sites at which a DNA fragment and a plasmid can be joined end to end, thereby forming a new plasmid that contains a piece of foreign DNA. The combined DNA formed by fusing a DNA fragment and a plasmid consists of parts from different kinds of organisms. In genetic engineering, molecules of combined DNA are known as chimeras because they are produced by combining DNA from different species. Combined DNA is also known as recombinant DNA, since DNA from two sources has been recombined to produce it.
The type of recombinant bacteria most often used to mass produce genes is?
I know of one commonly used bacteria: E. Coli
Why do you often use JM109 strain of E. coli as lab strain?
E.coli JM 109 is the most preferred strain in carrying out recombinant DNA techniques as it known to possess a stable genotype. This Strain also contains a F`episome which helps in blue/white screening and ss DNA rescue . It is used for the preparation of ssDNA , gene library construction and also cloning.
What role does E. coli play in recombinant DNA Technology?
It helps to create human insulin.
How did Boyer and Cohen performe their recombinant DNA experiment?
Herbert Boyer and Stanley Cohen created the first recombinant DNA organism using recombinant DNA technology, or gene splicing, which allows the manipulation of DNA. They showed that the gene for a frog ribosomal RNA could be transferred and expressed in bacterial cells. Boyer and Cohen removed plasmids, small rings of DNA located in a cell's cytoplasm, from a cell. Using restriction enzymes, they cut the DNA at precise positions and then recombined the DNA strands in their own way using DNA ligase enzyme. They then inserted the altered DNA into E. coli bacteria. The bacterial cells could be made to produce specific proteins using gene splicing. This technology was a major breakthrough for genetic engineering. Their experiments dramatically demonstrated the potential impact of DNA recombinant engineering on medicine and pharmacology, industry and agriculture.
What is recombinant DNA technology?
recombinant DNA is a form of artificial DNA which is engineered through the combination or insertion of one or more DNA strands,there by combining DNA sequences which would not normally occur together.
Is insertion of the insulin gene in E. coli an example of gene therapy?
No, its a good example of genetic engeneering though. To be more specific, it is an example of recombinant DNA technology.
Why is E. coli a prokaryotic cell?
Prokaryotes (as their name suggests) refer to cells which do not have a true membrane-bound nucleus. This type of cell includes most bacteria. As E. coli belongs to this category [its genetic material which includes genomic DNA (in the form of single-stranded DNA) and plasmid DNA (small circular DNA molecules in the cytoplasm of the cell) for some strains of E. coli e.g. DH5a] is sequestered in the cytoplasm of the cell in a region known as the nucleoid (rather than a true membrane bound nucleus), we may thus classify E. coli to be a prokaryotic cell.
What are two types of vector used in recombinant DNA technology?
there are many different vectores as: 1-plasmid system 2-bacteria phage lamda 3-cosmids 4-bacterio artificial system 5-puc system the other cloning vectors are m13 which is the oldest one. and after the above all are:- BAC(bacterial artificial chromosome) YAC(yeat artificial chromosome) TAC(transformation-competent artificial chromosome)
Is E. coli or salmonella worse?
e coli
What is a circular DNA strand used in recombinant DNA?
It is called a plasmid. Plasmids are extrachromosomal self-replicating circular forms of DNA present in most bacteria, such as E. Coli, containing genes related to catabolism and metabolic activity,and allowing the carrier bacterium to survive and reproduce in conditions present within other species and environments.
What is recombination DNA?
== == DNA fragments cannot function all by themselves. They must become part of the genetic material of living cells before the gene they contain can be activated. In the second step of genetic engineering, DNA fragments are made into part of the recipient cell's genetic material. This is done by combining DNA fragments with DNA from the recipient cell. For example, DNA fragments may be combined with bacterial DNA so that they can later be inserted into a bacterial cell. Bacteria often contain small circular DNA molecules known as plasmids in addition to their chromosomes. These plasmids can be removed from bacterial cells and cut with the same restriction enzyme used to produce the DNA fragments. The cuts made by the restriction enzyme produce matching "sticky ends" on the DNA fragments and the cut plasmids. These sticky ends are the sites at which a DNA fragment and a plasmid can be joined end to end, thereby forming a new plasmid that contains a piece of foreign DNA. The combined DNA formed by fusing a DNA fragment and a plasmid consists of parts from different kinds of organisms. In genetic engineering, molecules of combined DNA are known as chimeras because they are produced by combining DNA from different species. Combined DNA is also known as recombinant DNA, since DNA from two sources has been recombined to produce it.
Does salmonella have organelles?
No. Escherichia coli (E. coli) is a bacterial species. Bacteria differ from eukaryotes (such as animals, plants, and fungi) in that the chromosome is not surrounded by a membrane. In eukaryotes the nuclear membrane surrounds the DNA thus creating a compartment in the cell known as the nucleus.
What is an example of prokaryotic cell?
bacteria - salmonella - E. coli
Does E-coli cause Salmonella?
No. They are two different bacteria.
