WE do not know what effect you are asking about, but enzymes are proteins that act as catalysts, they speed up chemical reactions.
Once you boil the enzyme, it will be inactivated. Milk will have no effects of the enzyme.
Amylase (ptyalin)
Side effects associated with enzyme therapy include heartburn, nausea and vomiting, diarrhea, bloating, gas, and acne.
Physical activity can alter the shape of enzyme which can cause damage or may the enzyme become inactive
bacteria as many useful effects like it is used in the agriculture it is used in medicine to manufacture antibiotic some bacteria are live in alimentary canal to produce enzyme like CELLULASE to digest cellulose in herbivorus
Restriction enzymes.
pancreas
In enzyme-catalyzed reactions, the term "substrate" is synonymous with the molecule or molecules that the enzyme acts upon to produce a reaction. It is the specific substance upon which an enzyme acts to produce a product.
Parietal cells in the stomach lining produce pepsinogen, the precursor enzyme of hydrochloric acid. Pepsinogen is converted into pepsin, the active enzyme that helps break down proteins in the stomach.
Protease is a protein-degrading enzyme produced in the pancreas.
The enzyme luciferase works in conjunction with ATP to produce bioluminescence. Luciferase catalyzes the oxidation of luciferin in the presence of ATP, resulting in light production.
The enzyme function would not be as effecient, causing the liver to produce more enzymes.