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By adding a specific reagent.
So that you can measure how far it traveled.
Locating agent is a substance/liquid that help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.
A chromatogram is like a chemical fingerprint because a fingerprint is diffrent for every single person in the world and to indicate who is who we use fingerprints, for chromatography its a bit diffrent because you can have more than 2 types of the same thing. For example if someone has been killed & they found a little ink next ti the person, they would collect the ink. Then they would find the few people they think are the suspects. They would take a bit of each suspects' ink and then they would use chromatography to indicate who did it (who has the same ink as the murdurer ). So basically a chromatogram is like a chemical fingerprint because they both indicate who is who or who did what.
Towards the centre of the chromatogram. The smaller molecules are more mobile and appear towards the outside of the chromatogram.
During the development of a chromatogram, it is better to cover it with a beaker to prevent evaporation, which will affect the movement of the solute and solvent system. This will ensure that the chromatogram will develop properly.
Two ways of identifying amino acids on a chromatogram are paper and gas.
you spelling wrong wrong
The chromatogram, or chromatography paper has solvent on it. Each pigment will separate itself from the others due to the relative solubility of that particular pigment.
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By adding a specific reagent.
it is because sweat and other substances especially colored ones may be present there. so it is best not to touch chromatogram papers to aviod contaminating it and getting erroneous results..:) chem.student..:)
Resorcinol is a colouring agent for some chromatography experiments.
Bad things happen. The mobile phase evaporates and thus it's composition changes. The vapor phase above the chromatogram is not saturated or at equilibrium, and this affects the movement of the sample on the solid phase.
It help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.