0
How is he melting curve of duplex DNA affected by adding a small amount of ethanol?
Wiki User
∙ 12y ago
If a DNA strand is denatured, it is generally as a result of an increase in temperature. If monitored, the absorbance can be graphed as a function of temperature. The midpoint in the resulting curve is the melting point.
The melting curve can be altered by decreasing the ionic concentration, causing an decrease in the melting temperature. This is due to the Poly-anion nature of the DNA helix. When the ionic strength is decreased, the stability of the DNA strand decreases.
When a small amount of ethanol is added, the non-polar effect has the biggest impact. When EtOH is added, the non-polar nature of the solution is decreased, resulting in a decreased importance of the hydrophobic forces on the stability of the helix, resulting in a lower melting temperature.
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
What do you do if you drink ethanol?
Ethanol is another name for alcohol. Depending on amount and concentration, you may get drunk.
What volume of ethanol 96 percent should be added to 500 ml of 70 percent ethanol such that when the mix is made to 2.5l with water the final conc of ethanol is 55 percent?
First calculate how much ethanol you need in the final solution. 55% X 2.5l = 1.375l Then calculate how much ethanol you already have in the solution. 0.5l X 70% = 0.35l Now take the difference between those two numbers as the extra amount needed. 1.375l - 0.35l = 1.025l Now correct for the fact that you are adding only 96% ethanol. 1.025l / 96% = 1.068l Answer: 1.068l
Which material would require the least amount of energy to change 1kg from a solid to a liquid ethanol aluminum propane or water?
ethanol
Which one is used as antifreeze methanol or ethanol?
You can drink ethanol. If you drink methanol, even a small amount, you will go blind.
What is a major drawback of ethanol?
Two drinks and I have to get a taxi home. As an energy source, the process of concentrating ethanol consumes a lot of energy. It depends on who you believe, but the amount of energy consumed may exceed the amount of energy in the ethanol created. If that's so, then ethanol may be a useful form of energy (in the same was as electricity is), but not a useful source of energy.
When is water a solute?
We normally think of a solute as a solid that is added to a solvent (e.g., adding table salt to water), but the solute could just as easily exist in another phase. For example, if we add a small amount of ethanol to water, then the ethanol is the solute and the water is the solvent. If we add a smaller amount of water to a larger amount of ethanol, then the water could be the solute!
What is the amount of ethanol produced from 1kg of glucose?
1kg of glucose produce 0.5kg of ethanol
What limits the amount of synthetic ethanol produced?
the amount of feedstock avalialble
What do you do if you drink ethanol?
Ethanol is another name for alcohol. Depending on amount and concentration, you may get drunk.
How does the amount of ethanol affect crystal growth?
it doesnt
Why does solids have definite volume?
A solid has a definite volume because the molecules are tightly packed and whether it is an ice cube and turns into a liquid by melting it still is the same amount unless you are adding on to it.
What volume of ethanol 96 percent should be added to 500 ml of 70 percent ethanol such that when the mix is made to 2.5l with water the final conc of ethanol is 55 percent?
First calculate how much ethanol you need in the final solution. 55% X 2.5l = 1.375l Then calculate how much ethanol you already have in the solution. 0.5l X 70% = 0.35l Now take the difference between those two numbers as the extra amount needed. 1.375l - 0.35l = 1.025l Now correct for the fact that you are adding only 96% ethanol. 1.025l / 96% = 1.068l Answer: 1.068l
How does adding it subtracting a constant amount by each value in a set of data affect the mean?
When adding and subtracting a constant amount means that that amount will increase. The amount will increase dew to adding each number.
Which material would require the least amount of energy to change 1kg from a solid to a liquid ethanol aluminum propane or water?
ethanol
Which one is used as antifreeze methanol or ethanol?
You can drink ethanol. If you drink methanol, even a small amount, you will go blind.
What is a major drawback of ethanol?
Two drinks and I have to get a taxi home. As an energy source, the process of concentrating ethanol consumes a lot of energy. It depends on who you believe, but the amount of energy consumed may exceed the amount of energy in the ethanol created. If that's so, then ethanol may be a useful form of energy (in the same was as electricity is), but not a useful source of energy.
What increase absorbance of phenol in ethanol?
Increasing the amount of phenol in the solution
