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How is he melting curve of duplex DNA affected by adding a small amount of ethanol?
Wiki User
∙ 12y ago
If a DNA strand is denatured, it is generally as a result of an increase in temperature. If monitored, the absorbance can be graphed as a function of temperature. The midpoint in the resulting curve is the melting point.
The melting curve can be altered by decreasing the ionic concentration, causing an decrease in the melting temperature. This is due to the Poly-anion nature of the DNA helix. When the ionic strength is decreased, the stability of the DNA strand decreases.
When a small amount of ethanol is added, the non-polar effect has the biggest impact. When EtOH is added, the non-polar nature of the solution is decreased, resulting in a decreased importance of the hydrophobic forces on the stability of the helix, resulting in a lower melting temperature.
Wiki User
∙ 12y ago
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Does the pH of ethanol change if water is added?
Yes, adding water to ethanol will change its pH. Ethanol is a neutral compound with a pH of around 7. However, adding water will dilute the ethanol, potentially changing its pH depending on the amount of water added and the initial concentration of the ethanol solution.
What is the molar heat of fusion of ethanol?
The molar heat of fusion of ethanol is approximately 5.02 kJ/mol. This is the amount of energy required to convert one mole of solid ethanol into liquid ethanol at its melting point of -114.1°C.
Which material would require the least amount of energy to change 1kg from a solid to a liquid ethanol aluminum propane or water?
Water would require the least amount of energy to change 1kg from a solid to a liquid because it has a lower melting point compared to the other materials listed (ethanol, aluminum, propane).
Does the melting rate depend on the amount of solute added?
Yes, adding a solute to a solvent can affect the melting rate. When a solute is added, it disrupts the crystal lattice structure of the solvent, which can either increase or decrease the melting rate depending on the specific solute-solvent interaction. Generally, adding a solute lowers the melting point of the solvent and can lead to a faster melting rate.
Lower the melting point of wax?
To lower the melting point of wax, you can consider adding a small amount of mineral oil or vegetable oil to the wax before melting it. This will decrease the overall melting temperature of the wax. Alternatively, you can mix the wax with a lower melting point wax to create a blend with a reduced melting point.
Does the pH of ethanol change if water is added?
Yes, adding water to ethanol will change its pH. Ethanol is a neutral compound with a pH of around 7. However, adding water will dilute the ethanol, potentially changing its pH depending on the amount of water added and the initial concentration of the ethanol solution.
What is the molar heat of fusion of ethanol?
The molar heat of fusion of ethanol is approximately 5.02 kJ/mol. This is the amount of energy required to convert one mole of solid ethanol into liquid ethanol at its melting point of -114.1°C.
Which material would require the least amount of energy to change 1kg from a solid to a liquid ethanol aluminum propane or water?
Water would require the least amount of energy to change 1kg from a solid to a liquid because it has a lower melting point compared to the other materials listed (ethanol, aluminum, propane).
When is water a solute?
We normally think of a solute as a solid that is added to a solvent (e.g., adding table salt to water), but the solute could just as easily exist in another phase. For example, if we add a small amount of ethanol to water, then the ethanol is the solute and the water is the solvent. If we add a smaller amount of water to a larger amount of ethanol, then the water could be the solute!
Does the melting rate depend on the amount of solute added?
Yes, adding a solute to a solvent can affect the melting rate. When a solute is added, it disrupts the crystal lattice structure of the solvent, which can either increase or decrease the melting rate depending on the specific solute-solvent interaction. Generally, adding a solute lowers the melting point of the solvent and can lead to a faster melting rate.
What is the amount of ethanol produced from 1kg of glucose?
1kg of glucose produce 0.5kg of ethanol
What limits the amount of synthetic ethanol produced?
The availability and cost of feedstock (such as corn or sugar cane) needed for ethanol production, as well as the efficiency of the conversion process, can limit the amount of synthetic ethanol produced. Additionally, factors like government regulations, market demand, and competition with fossil fuels can also impact the production of synthetic ethanol.
Is sodium sulfate soluble in ethanol?
Sodium sulfate is slightly soluble in ethanol. At room temperature, only a small amount of sodium sulfate will dissolve in ethanol.
Why does solids have definite volume?
A solid has a definite volume because the molecules are tightly packed and whether it is an ice cube and turns into a liquid by melting it still is the same amount unless you are adding on to it.
Lower the melting point of wax?
To lower the melting point of wax, you can consider adding a small amount of mineral oil or vegetable oil to the wax before melting it. This will decrease the overall melting temperature of the wax. Alternatively, you can mix the wax with a lower melting point wax to create a blend with a reduced melting point.
How does adding it subtracting a constant amount by each value in a set of data affect the mean?
When adding and subtracting a constant amount means that that amount will increase. The amount will increase dew to adding each number.
If ethanol has a density of 789 g then what is the mass of 225 g of ethanol?
The mass of 225 g of ethanol would be 225 g. The information given about the density of ethanol is not relevant to finding the mass of the given amount (225 g) of ethanol.
