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Insulin is released when blood glucose levels are too high. When there is a lot of glucose in the blood, it diffuses into the beta cells of the pancreas, and is used to produce ATP. This ATP causes the potassium channels in the cell membrane to close. Potassium ions cannot diffuse out of the cell, and so there is a smaller potential diffference across the cell's membrane, because they inside has become less negative (potassium ions have a positive charge).

The change in potential difference causes calcium channels in the beta cell membrane to open, and calcium ions diffuse in down their concentration gradient.

Inside the beta cells there are vesicles containing insulin. As calcium ions diffuse into the cell, they cause the vesicles to move towards the membrane, and fuse with it, releasing the insulin into the blood stream by exocytosis.

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