Two days at room temperature on TSA (tryptic soy agar)
A nutrient medium is used.
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Bacillus subtilis is grown on nutrient agar or in nutrient broth. The preferred medium is M9 with glucose.
They are grown on medium that contains the nutrient that they cannot synthesize
inoculation
No. The reason is, Chromogenic microorganisms may produce intracellular pigments that are responsible for the coloration of the organisms as seen in surface colonies. Other organisms produce extracellular soluble pigments that are excreted into the medium & that also produce a color. BUT........ most organisms, however, are nonchromogenic & will appear white to gray. ~jmata Microbiology 205 lab manual question. ~
A nutrient medium is used.
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simply agar medium
simply agar medium
Hydroponics substitute a nutrient medium in place of soil. for the plants to root and grow. The nutrient medium may be either a water solution or an inert material.
Bacillus subtilis is grown on nutrient agar or in nutrient broth. The preferred medium is M9 with glucose.
They are grown on medium that contains the nutrient that they cannot synthesize
Submerged fermentation:" The organism which can grow under the beneath the surface of the medium is called " Ex.. liquid medium (Nutrient broth) solid state-fermentation : "Organisms which can grow on the surface of the medium is called solid state fermentation" Ex.. solid medium (Nutrient agar)
Water
inoculation
In our lab we use LB agar, LB broth, and 2XYT broth.