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The fermentation pathway itself does not generate NADH itself. In fact NAD+ builds up. Glycolysis uses the NADH when oxidating carbon substrates and fermentation is used to regenerate the NAD+ and thus the cycle continues.

If fermentation did not exist, NADH would build up and the cell would not be able to oxidize carbon anymore. The cell would die.

In the case of respiration (aerobic or anaerobic) the cell will replenish its NAD+ pool the electron transport chain (oxidative phosphorylation). This generates even more potential to make ATP by pumping protons out of the cell using the energy generated from NADH -> NAD via NADH dehydrogenase. This gradient can be utilized by allowing the protons to flow back into the cell through ATPase, generating ATP. The utilization of NADH to pump protons out of the cell is the sole reason why respiration generates 36-38 ATP while fermentation generates 2 ATP per glucose.

After much rambling, the point to take home is that the main job of fermentation in the cell is the replenish the NAD+ pool so that glycolysis can continue which drives biosynthesis.

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12y ago
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11y ago

NADH is oxidized when it donates its H+ and electrons to pyruvate which becomes reduced.

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citric acid cycle - pyruvic acid

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Yes .

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12y ago

4 NADH

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Q: How many NADH are produced during fermentation?
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