266 miles taking this route: Take I-10 WEST from Jacksonville to I-75 SOUTH to TAMPA off EXIT 296A. Take I-75 SOUTH to I-275 SOUTH to TAMPA and ST. PETERSBURG off EXIT 274. Take I-275 SOUTH down to SR-60 WEST to CLEARWATER off EXIT 32. Take SR-60 WEST to Clearwater.
It is approximantly, 171 miles from Tampa Florida, to Jacksonville Florida. This is Road miles. Now in nautical miles it is approximantly, 141 miles. Jon McMullen FireFighter It is 200.65 road miles according to MapQuest.