How many people sailed with Columbus?

Columbus sailed with 90 men on his first voyage.

26 on the Pinta
24 on the Nina
and 40 on the Santa Maria

Santa Maria ran aground and sank and Columbus had to leave 39 men behind in the Caribean for his return journey. These men were all killed by the natives before Columbus returned on his second voyage.
Columbus took about 20 natives as slaves, only 7 of which survived the crossing to spain.