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"Reuse of Ni-NTA Resin

The reuse of Ni-NTA resin depends on the nature of the sample and should only be performed with identical recombinant proteins. Based on the experience of Hoffmann-La Roche Ltd. (Basel, Switzerland), who have purified more than 100 different proteins on Ni-NTA resin, we recommend a maximum of 5 runs per column."

The QIAexpressionist (QIAGEN, 2003)

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