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What Reagents are used in osazone test?
Reagents of Osazone test are 1. Phenylhydrazine mixture consisting of phenyl-hydrazine and anhydrous sodium citrate 2. Orignal solution containing a carbohydrate
What is the importance of phenylhydrazine test?
The phenylhydrazine test is also known as the Osazone test. The phenylhydrazine test is used for reducing the amounts of carbohydrates, momosacchstides, and disaccharides.
Osazone test for carbohydrates?
osazone test is a carbohydrate specific reaction in which carbs react with phenylhydrazine to form osazones i.e. crystals of specific shape. thus the test confirms the presence of a particular sugar.Eg:for sucrose one gets a mixture of glucosazone and fructosazone on the basis of components of sucrose.
What are two aldotetroses that yield the same osazone on reaction with phenyl hydrazine?
D-erythrose and D-threose both yield the same osazone. Likewise, L-erythrose and L-threose yield the same osazone.
Why glucose and fructose form same osazone?
Glucose, Fructose and Mannose give the same osazone because these sugars have the same configuration of carbons 3, 4, 5 and 6. osazone formation only affects carbons 1 and 2.
Why osazone obtained from D-glucose and D-fructose have the same melting points?
glucose and fructose form same osazone crystals because carbon no 1 to carbon no 6 are identical both in glucose and in fructose
Why you used sodium acetate in osazone test?
Anhydrous sodium acetate is added to buffer the phenylhydrazine HCl so that it may function properly. Regards mawaisnawaz@hotmail.com
How many phenylhydrazines are required in osazone reaction?
3
3reasons why the osazone test can be used in the identification of sugars?
1.due to their characteristic crystalline appearance 2.due to their definite melting point 3.due to the length of time for the crystals to form
3 reasons why the osazone test can be used in identification of sugars?
a. It is due to their characteristic crystalline appearance b. It is due to their definite melting point c. It is due to the length of time for the crystals to form
What is the clinical significance of osazone test?
For identification purposes the carbonyl and adjacent alcohol functions will form phenylhydrazine derivatives known as osazones, which give characteristic melting points and exhibit definite crystalline structure. It should be noted that glucose , fructose and mannose yield the same osazone since the difference in structure and configuration about carbon atoms 1 and 2 are abolished.
Why glucose and fructose form similar osazone crystals?
since carbons #3 through #6 of D-glucose and D-fructose molecules are identical, the sa,e osazone is formed.
