osazone test is a carbohydrate specific reaction in which carbs react with phenylhydrazine to form osazones i.e. crystals of specific shape. thus the test confirms the presence of a particular sugar.Eg:for sucrose one gets a mixture of glucosazone and fructosazone on the basis of components of sucrose.
D-erythrose and D-threose both yield the same osazone. Likewise, L-erythrose and L-threose yield the same osazone.
Glucose, Fructose and Mannose give the same osazone because these sugars have the same configuration of carbons 3, 4, 5 and 6. osazone formation only affects carbons 1 and 2.
The Antrone test is a general test for carbohydrates (like the Molisch test). Thus, all carbohydrates should yield a positive test (green precipitate) in the anthrone reaction.
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carbohydrates, for one
The phenylhydrazine test is also known as the Osazone test. The phenylhydrazine test is used for reducing the amounts of carbohydrates, momosacchstides, and disaccharides.
Reagents of Osazone test are 1. Phenylhydrazine mixture consisting of phenyl-hydrazine and anhydrous sodium citrate 2. Orignal solution containing a carbohydrate
i think the general test for carbohydrates is Molisch Test..
D-erythrose and D-threose both yield the same osazone. Likewise, L-erythrose and L-threose yield the same osazone.
Glucose, Fructose and Mannose give the same osazone because these sugars have the same configuration of carbons 3, 4, 5 and 6. osazone formation only affects carbons 1 and 2.
The Antrone test is a general test for carbohydrates (like the Molisch test). Thus, all carbohydrates should yield a positive test (green precipitate) in the anthrone reaction.
Because it is a carbohydrates and molisch test will be positive in the presence of carbohydrates
Molisch's Test is a chemical test for the presence of carbohydrates, based on the dehydration of the carbohydrate by sulfuric acid to produce an aldehyde. Its importance is that it tests the presence of carbohydrates based on their dehydration,
Bread, pasta, and cereal would certainly "test positive" for carbohydrates.
glucose and fructose form same osazone crystals because carbon no 1 to carbon no 6 are identical both in glucose and in fructose
Anhydrous sodium acetate is added to buffer the phenylhydrazine HCl so that it may function properly. Regards mawaisnawaz@hotmail.com
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