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The biuret test is a chemical test used for detecting the presence of peptide bonds. In the presence of peptides, a copper(II) ion forms violet-colored coordination complexes in an alkaline solution.[1] Several variants on the test have been developed.
The Biuret reaction can be used to assay the concentration of proteins because peptide bonds occur with the same frequency per amino acid in the peptide. The intensity of the color, and hence the absorption at 540 nm, is directly proportional to the protein concentration, according to the Beer-Lambert law.
Sigurd Nolan
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What are the risks of testing unknown solutions and how might you manage these risks?
any unknown solution may be poisinous , highly acidic that may burn ur tongue etc. hence without verification of any soln it is dangerous to taste it directly. their are many ways of tasting the properties of soln like litmus test, hydrogen test , ph test, metal test.. etc thanx
You are given two test tubes and told that one contains a protein and the other amino acid. Explain fully how you would identify the test tube that contains amino acid?
This may be wrong but I think you could use the biuret test as this would only give a positive result in the test tube with protein in
Why do you use a water bath to heat the mixture in Benedict's test for glucose and millon's test for protein?
because the protein
How amylase breakdown starch?
To test starch: To test starch you take the food sample, and add iodine solution if the colour turns black this means starch is present. To test for protein: To test for protein, you take the food sample and add Biuret A and Biuret B and shake, if the colour turns lilac this means that protein is present.
How do you identify a unknown white powder?
Thomas, but his friends just call him Tom.
What test indicator is used to detect protein?
To test for protein. Biuret or solutions of sodium hydroxide and copper sulfate. Tested with a dropper a + result will be purple or something
How would you test an unknown solution for each of the following sugar fat starch protein?
Sugars - Benedict's solution Fats - Brown Paper Starch - Iodine Protein - Biuret Solution
Why would you test pure water in a calorimeter to find the concentration of protein in a solution?
test the pureity of water
What color is a positive test for protein?
A positive test, when using Biuret reagent, would be purple-ish
Which is the hexane layer?
In a Halide Test, you can add an unknown solution into a test tube and add hexane solution plus several other solutions called by the procedure. Shake the test tube and record the upper hexane layer. So the answer would be hexane is present in the upper layer.
What is a chemical test for protein?
You can use hydrogen peroxide to test for protein>
What are the risks of unknown solutions and how might you manage these risks?
any unknown solution may be poisinous , highly acidic that may burn ur tongue etc. hence without verification of any soln it is dangerous to taste it directly. their are many ways of tasting the properties of soln like litmus test, hydrogen test , ph test, metal test.. etc thanx
What are the risks of testing unknown solutions and how might you manage these risks?
any unknown solution may be poisinous , highly acidic that may burn ur tongue etc. hence without verification of any soln it is dangerous to taste it directly. their are many ways of tasting the properties of soln like litmus test, hydrogen test , ph test, metal test.. etc thanx
Why does albumin and pepsin test positive for protein?
Albumin and pepsin are both proteins, the test for proteins was positive.
How can I find out whether a unknown mixture has protein in it?
The most common method is the biuret test: in the presence of a copper(II) solution and NaOH the sample become violet.
What is the chemical indicator used to test protein?
To test for protein. Biuret or solutions of sodium hydroxide and copper sulfate. Tested with a dropper a + result will be purple or something
Where can i find Numerical aptitude test questions and solutions?
You cannot find the solutions to the questions, but the best website to give you practice would be the SHL website.
