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If e. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure what would happen?
Wiki User
∙ 15y ago
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
Wiki User
∙ 15y ago
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If E. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure then will reaction be carried out in waterbath?
no e coli DNA pol can not with stand high temperature if so for every denaturation step we must add fresh enzyme
What is the explanation for the Role of RNA polymerase?
Basically, RNA polymerase's role is very similar to that of DNA polymerase. RNA polymerase is an enzyme that is used during transcription in the nucleus. Similar to DNA polymerase, RNA polymerase codes for the complementary nucleotides to a DNA strand. Instead of thymine though, uracil codes with adenine. This coded mRNA strand then travels from the nucleus to the ribsome where translation occurs - the result is protein made from an amino acid chain. To answer your main question - RNA polyermase adds the complementary nucleotides to the DNA strand using uracil instead of thymine. hope that helps :)
What endothermic reaction?
An endothermic reaction is a chemical reaction in which energy (heat, light, etc.) is absorbed instead of released as in a exothermic reaction.
Differences between DNA polymerase and RNA polymerase?
A polymerase is an enzyme that catalyzes the conversion of free nucleotides into a single strand. DNA polymerase differs from RNA polymerase in two major respects: * Like all enzymes, DNA polymerase is substrate-specific. DNA polymerase cannot extend a single strand of DNA; it needs at least a short segment of double-stranded DNA at the outset. * As its name implies, DNA polymerase incorporates deoxyribonucleotides into the new strand. RNA polymerase incorporates ribonucleotides. These differences mean that DNA polymerase is active when new DNA strands are formed, as in DNA replication, and RNA polymerase is active when new RNA is formed, as in transcription. Before DNA replication can begin, the two strands must uncoil, so that each can form a template for free nucleotides to attach to. But DNA polymerase cannot get started with a single strand! In vivo(in the cell) RNA polymerase, which is active in the presence of single-stranded DNA, catalyzes the incorporation of a handful of nucleotides into a new strand. The short length of double-stranded nucleic acid that is produced enables DNA polymerase to swing into action. This still leaves a potential difficulty: the nucleotides incorporated in the presence of RNA polymerase are the wrong sort (ribonucleotides). They are subsequently replaced by DNA polymerase. In vitro (during PCR, the polymerase chain reaction) a primer, specially synthesized in a laboratory, attaches to a specific segment of single-stranded DNA, and the DNA polymerase takes over from there. The primer consists of a short length of single-stranded DNA that uniquely complements a specific DNA segment that is targeted for amplification, for example for forensic analysis.In practice, there are several different DNA polymerases and RNA polymerases in an organism.
What is a strictureplasty?
It is a procedure that widens the intestine instead of making it shorter
Why rna primer is used instead of dna primer?
There are basically two types of enzymes that can bind to DNA and copy it. The DNA polymerase and the RNA polymerase. The RNA polymerase, which copies DNA into RNA, will only bind to single stranded DNA, in other words areas of the DNA where the nitrogen bases holding the two strands of nucleotide units together have been separated. On the other hand the DNA polymerase that copies DNA into DNA will only bind to DNA that is double stranded. So in lies the dilemma. To make a copy of the DNA the DNA polymerase is use, but it will not bind to single stranded DNA so there is no way to make a DNA primer using aDNA polymerase, but the RNA polymerase will bind to single stranded DNA and there for can be used to make a small RNA primer on the open strands of DNA. Now the DNA polymerase has place that is double stranded and can attach and start copying the DNA.
Is vapour a chemical reaction?
Vapour is not a chemical reaction; instead, it is a tangible substance in a gaseous state and has no necessary association with chemical reaction at all.
What other words can be used instead of a process?
Progression,progress ,proceeding ,procedure
Explain the role of RNA polymerase in mRNA sythesis?
During transcription, RNA Polymerase binds with upstream of the gene that is to be transcribed into mRNA. This causes the double helix shape of the DNA to unwind. Then, RNA Polymerase uses the template strand of the DNA as guide and synthesizes a complementary mRNA strand using ribonucleotides. Basically, without RNA Polymerase, mRNA would not be synthesized.
Why not do a Hysterectomy instead of LEEP procedure?
When the problem on the cervix is dysplasia, and not cancer, the risk of the hysterectomy outweighs the benefit.
Which minimally invasive procedure may be used instead of TURP for the treatment of BPH?
Green-light PVP
Is not a reflection but is instead a reaction?
reading Death of a Salesman made me angry at willy loman
