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What else can I help you with?
If E. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure then will reaction be carried out in waterbath?
No, the reaction will not be carried out in a water bath. E. coli DNA polymerase requires higher temperatures to function optimally for PCR, typically around 72°C. Therefore, a thermal cycler with the ability to cycle through different temperatures is needed to perform PCR with E. coli DNA polymerase.
Why human DNA polymerase cannot be used in PCR technique?
Human DNA polymerase cannot be used in PCR (Polymerase Chain Reaction) because it is sensitive to high temperatures required for denaturation of DNA, which can lead to its denaturation and loss of activity. PCR involves repeated cycles of heating and cooling, and traditional DNA polymerases would not withstand these conditions. Instead, thermostable DNA polymerases, such as Taq polymerase from Thermus aquaticus, are used because they remain functional at high temperatures, allowing for efficient amplification of DNA.
Why normal DNA polmerase not use in PCR?
In the PCR, high temperatures are used in order to separate both strands of DNA readily. Normal DNA polymerases would "melt" (denature) under these conditions, whereas Taq DNA Polymerase does not (short from Thermus aquaticus, a bacteria that lives in very hot submarine springs).
What is the explanation for the Role of RNA polymerase?
Basically, RNA polymerase's role is very similar to that of DNA polymerase. RNA polymerase is an enzyme that is used during transcription in the nucleus. Similar to DNA polymerase, RNA polymerase codes for the complementary nucleotides to a DNA strand. Instead of thymine though, uracil codes with adenine. This coded mRNA strand then travels from the nucleus to the ribsome where translation occurs - the result is protein made from an amino acid chain. To answer your main question - RNA polyermase adds the complementary nucleotides to the DNA strand using uracil instead of thymine. hope that helps :)
How does RNA polymerase contribute to the process of DNA replication?
RNA polymerase is not directly involved in DNA replication. Instead, it is responsible for transcribing DNA into RNA during the process of gene expression. DNA replication is carried out by a different enzyme called DNA polymerase, which synthesizes new DNA strands using the existing DNA as a template.
What role does RNA polymerase play in the process of DNA replication?
RNA polymerase is not directly involved in DNA replication. Instead, it is responsible for transcribing DNA into RNA during the process of gene expression. DNA replication is carried out by a different enzyme called DNA polymerase, which synthesizes new DNA strands using the existing DNA as a template.
What endothermic reaction?
An endothermic reaction is a chemical reaction in which energy (heat, light, etc.) is absorbed instead of released as in a exothermic reaction.
What is a strictureplasty?
It is a procedure that widens the intestine instead of making it shorter
Differences between DNA polymerase and RNA polymerase?
A polymerase is an enzyme that catalyzes the conversion of free nucleotides into a single strand. DNA polymerase differs from RNA polymerase in two major respects: * Like all enzymes, DNA polymerase is substrate-specific. DNA polymerase cannot extend a single strand of DNA; it needs at least a short segment of double-stranded DNA at the outset. * As its name implies, DNA polymerase incorporates deoxyribonucleotides into the new strand. RNA polymerase incorporates ribonucleotides. These differences mean that DNA polymerase is active when new DNA strands are formed, as in DNA replication, and RNA polymerase is active when new RNA is formed, as in transcription. Before DNA replication can begin, the two strands must uncoil, so that each can form a template for free nucleotides to attach to. But DNA polymerase cannot get started with a single strand! In vivo(in the cell) RNA polymerase, which is active in the presence of single-stranded DNA, catalyzes the incorporation of a handful of nucleotides into a new strand. The short length of double-stranded nucleic acid that is produced enables DNA polymerase to swing into action. This still leaves a potential difficulty: the nucleotides incorporated in the presence of RNA polymerase are the wrong sort (ribonucleotides). They are subsequently replaced by DNA polymerase. In vitro (during PCR, the polymerase chain reaction) a primer, specially synthesized in a laboratory, attaches to a specific segment of single-stranded DNA, and the DNA polymerase takes over from there. The primer consists of a short length of single-stranded DNA that uniquely complements a specific DNA segment that is targeted for amplification, for example for forensic analysis.In practice, there are several different DNA polymerases and RNA polymerases in an organism.
Is an enhancer is a sequence of nucleotides that when activated by specific signal proteins aids in shielding the RNA polymerase binding site of a specific gene?
An enhancer is a DNA sequence that functions to increase the transcription of a gene by facilitating the binding of transcription factors and RNA polymerase to the promoter region. It does not shield the RNA polymerase binding site; instead, it enhances gene expression by increasing the rate of transcription.
What other words can be used instead of a process?
Progression,progress ,proceeding ,procedure
What is a primer dimer?
A primer dimer is a byproduct that can occur during the polymerase chain reaction (PCR) when two primers anneal to each other instead of to the target DNA sequence. This can lead to the amplification of non-target products, reducing the efficiency and specificity of the PCR reaction. Primer dimers typically manifest as shorter DNA fragments and can interfere with the desired amplification of the target DNA. Proper primer design and optimization can help minimize the formation of primer dimers.
