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Human DNA polymerase cannot be used in PCR (Polymerase Chain Reaction) because it is sensitive to high temperatures required for denaturation of DNA, which can lead to its denaturation and loss of activity. PCR involves repeated cycles of heating and cooling, and traditional DNA polymerases would not withstand these conditions. Instead, thermostable DNA polymerases, such as Taq polymerase from Thermus aquaticus, are used because they remain functional at high temperatures, allowing for efficient amplification of DNA.
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What organism is used primarily in PCR technique?
The organism used primarily in PCR (polymerase chain reaction) technique is a heat-stable DNA polymerase, such as Taq polymerase. Taq polymerase is derived from the thermophilic bacterium Thermus aquaticus, which can withstand the high temperatures required for PCR amplification.
What is the source of the polymerase used in polymerase chain reaction?
The polymerase used in polymerase chain reaction (PCR) is typically derived from a thermophilic bacterium called Thermus aquaticus. The specific polymerase most commonly used is Taq polymerase, which is known for its ability to withstand high temperatures required for PCR.
PCR stands for?
Polymerase chain reaction. It is a technique used in molecular biology to amplify a specific DNA sequence. It involves cycles of heating and cooling to produce millions of copies of a particular DNA fragment.
What is the technique used for studying the human body?
To study the human body we use touch, tensioned
In order to replicate DNA outside living organisms scientists use?
Polymerase chain reaction (PCR) is a widely used technique that allows scientists to amplify specific DNA sequences in a test tube. This process involves repeatedly heating and cooling the DNA to facilitate replication. Another method is isothermal amplification, which amplifies DNA at a constant temperature using enzymes like Bst polymerase.
What organism is used primarily in PCR technique?
The organism used primarily in PCR (polymerase chain reaction) technique is a heat-stable DNA polymerase, such as Taq polymerase. Taq polymerase is derived from the thermophilic bacterium Thermus aquaticus, which can withstand the high temperatures required for PCR amplification.
What is the source of the polymerase used in polymerase chain reaction?
The polymerase used in polymerase chain reaction (PCR) is typically derived from a thermophilic bacterium called Thermus aquaticus. The specific polymerase most commonly used is Taq polymerase, which is known for its ability to withstand high temperatures required for PCR.
PCR stands for?
Polymerase chain reaction. It is a technique used in molecular biology to amplify a specific DNA sequence. It involves cycles of heating and cooling to produce millions of copies of a particular DNA fragment.
Which best describes the purpose of Polymerase chain reaction?
Polymerase chain reaction (PCR) is a technique used to make millions of copies of a specific segment of DNA. It is primarily used for DNA amplification, allowing for the detection and analysis of small amounts of DNA, such as in forensic testing, genetic research, and medical diagnostics.
What is the technique used for studying the human body?
To study the human body we use touch, tensioned
What does the medical abbreviation PCR mean?
PCR stands for "polymerase chain reaction," which is a molecular biology technique used to amplify and detect specific DNA sequences. It is commonly used in medical diagnostics and research to detect viruses, bacteria, and genetic mutations.
What is the language technique used in the phrase the cold within him froze his old features?
The language technique used in this phrase is personification, where human characteristics are attributed to something non-human. Here, the cold is given the ability to freeze, which is a human action.
Is the enzyme that allows replication to occur DNA polymerase or RNA polymerase?
DNA polymerase replicated DNA. RNA polymerase creates mRNA to be used in protein synthesis. RNA polymerase does not replicated DNA.
Which best describes the purpose of the polymerase chain reaction (PCR)?
Polymerase Chain Reaction is widely used in many areas to identify DNA and detect infectious organisms or genetic variations, including the viruses that cause AIDS, hepatitis, and tuberculosis, detection of mutations in human genes, and numerous other tasks.
Is used to make multiple copies of a particular gene?
The Polymerase Chain Reaction (PCR) technique employs a heat-stable polymerase in a chain reaction, replicating DNA exponentially.
Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
What is defined as rt pcr?
RT-PCR stands for reverse transcription polymerase chain reaction. It is a molecular biology technique used to amplify and quantify RNA molecules by converting them into complementary DNA (cDNA) and then amplifying the cDNA using PCR. RT-PCR is commonly used in gene expression analysis, viral detection, and diagnostic testing.
