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If you have performed a transformation experiment using a mutant strain that is arg-- trp plus gal-- his--.?
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∙ 6y ago
You have performed a transformation experiment using a mutant strain that is arg-- trp+ gal-- his--. A culture of this mutant strain was mixed with a DNA from another mutant strain that is arg+ trp-- gal+ his+.
How will you select for the following recombinants?
a) arg+ trp+ gal-- his--
b) arg+ trp-- gal+ his+
c) arg-- tryp-- gal+ his--
d) arg-- trp+ gal-- his+
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∙ 6y ago
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How are cells containing recombinant DNA selected?
You have performed a transformation experiment using a mutant strain that is arg-- trp+ gal-- his--. A culture of this mutant strain was mixed with a DNA from another mutant strain that is arg+ trp-- gal+ his+. How will you select for the following recombinants? a) arg+ trp+ gal-- his-- b) arg+ trp-- gal+ his+ c) arg-- tryp-- gal+ his-- d) arg-- trp+ gal-- his+
What was the molecule that caused transformation in Griffith's pneumonia experiment?
A molecue called the s (strain).
Can blending denature enzymes in papaya?
NO! As you can see many lab require you to blend the papaya then strain it to properly conduct the experiment :)
What is most likely to occur in a patient infected with a mutant strain of B pertussis that lacks functional adhesins?
The bacterium would be less likely to colonize the patient's lower respiratory system.
What important question remained unanswered by griffith experiment?
Assuming you mean the Frederick Griffith bacterial experiment, the question left unanswered was how the rough strain (less harmful) bacteria transformed into the smooth strain (lethal) bacteria. When he injected live rough strain bacteria, the mouse lived. When he injected live smooth strain bacteria, the mouse died. But if dead smooth strain bacteria was injected, the mouse lived. So if either live rough strain or dead smooth strain could be injected without killing the mouse, then it would stand to reason that one could inject both, the mouse should live. But the mouse died. So he figured the rough strain was somehow taking on the characteristics of the smooth strain bacteria, perhaps by being in close proximity to dead smooth strain bacteria, but he didn't know why. We now know that the smooth strain DNA was somehow getting grafted into the rough strain bacteria and making it able to create a coating which prevented the immune system (of the mouse in this case) from killing it. In case you mean the John Howard Griffin racial change experiment, the question left unanswered was how to stop racism. While his experiment was not completely successful, he gained a number of insights into the experiences of men of color in America. He was able to speak to people in both groups, but yet, he was not able at that point to get them to reconcile with each other.
How are cells containing recombinant DNA selected?
You have performed a transformation experiment using a mutant strain that is arg-- trp+ gal-- his--. A culture of this mutant strain was mixed with a DNA from another mutant strain that is arg+ trp-- gal+ his+. How will you select for the following recombinants? a) arg+ trp+ gal-- his-- b) arg+ trp-- gal+ his+ c) arg-- tryp-- gal+ his-- d) arg-- trp+ gal-- his+
What was the molecule that caused transformation in Griffith's pneumonia experiment?
A molecue called the s (strain).
What do you call pieces of DNA that are put together?
Engineered strain or mutant strain. If they are to be propagated in an organism, the mode of transformation/transfection is called a vector. Target DNA + vector = charged vector.
How does Frederick Griffith's experiment demonstrate transformation?
Frederick Griffith's experiment showed that genetic material could be transferred between different strains of bacteria, leading to a change in their characteristics. This transfer of genetic material is known as transformation, which was demonstrated when the nonvirulent strain of bacteria was transformed into a virulent one after being in contact with genetic material from the virulent strain.
What is transformation in DNA?
transformation is the process in which one strain of bacteria is changed by a gene or genes from another strain of bacteria
The process by which one strain of bacteria is apparently changed into another strain is called what?
conjugation or transduction or translation are the three process by which bacteria can change from one form to other
What is the process in which one strain of bacteria changes into another?
transformation
Scientists performed an experiment to determine whether there is a connection between learning ability and food They took two groups of 20 mice each all from the same purebred strain The mice were?
The experiment could be repeated on the same mice.
Scientists performed an experiment to determine whether there is a connection between learning ability and food They took two groups of 20 mice each all from the same purebred strain The mice you?
the experiment could be repeated with the same mice
What did Griffith discover as a result of his experiments?
Frederick Griffith was a British bacteriologist. In January 1928, he reported what is now known as Griffith's Experiment, the first widely accepted demonstrations of bacterial transformation, whereby a bacterium distinctly changes its form and function.
What is it called when an object changes its size or shape?
When something changes like that it is called transformation.
Who discovered that the hereditary system of one strain of bacteria?
Frederick Griffith is credited with discovering the hereditary system involving transformation in bacteria. In his experiments with Streptococcus pneumoniae in 1928, Griffith observed that genetic material could be transferred between different strains of bacteria, influencing their traits. This discovery laid the foundation for understanding DNA as the hereditary material.
