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In preparation for electrophoresis procedure enzymes are added to DNA in order to?
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What happens when enzymes are added to starch suspension?
The enzyme eg. Amylase will break down the starch into sugar.
Why excess barium chloride is added to the filtrate in the preparation of a dry sample of barium sulphate?
Barium chloride in excess is added to be sure that the reaction is complete.
What substance is added durning hydrolysis in order to break food macromolecules?
Mainly enzymes are involved. They are made up of proteins
What is the cutting of DNA by specific enzymes known as restriction endonucleases the cut piece from one organism is added to the DNA of another?
GE
What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
What is the preparation for a trabeculectomy?
The procedure and its benefits and possible complications are fully explained. Antiglaucoma drugs are prescribed before surgery. Added pressure on the eye caused from coughing or sneezing should be avoided.
Why is citric added to juice drink preparation?
Citric acid is added for tartness.
When are enzymes added to your food?
the pancreas excretes enzymes in to the small intestine to break down your food
Why ammonia is added in preparation of EDTA solution?
To maintain pH=10
What happens when enzymes are added to starch suspension?
The enzyme eg. Amylase will break down the starch into sugar.
What is the work of NaCl in soap preparation?
Sodium chloride is added to precipitate the soap.
Why are enzymes mixed with vinegar less effective?
Enzymes are sensitive to changes in pH and only work with a relatively small pH range. Since vinegar is an acid, the pH of the solution containing the enzymes is changes when vinegar is added.
When reporting a staged procedure what modifier is added to the CPT code?
58
Why excess barium chloride is added to the filtrate in the preparation of a dry sample of barium sulphate?
Barium chloride in excess is added to be sure that the reaction is complete.
Why are enzymes added to laundry detergent?
The enzymes used in laundry detergents act on materials that make up a variety of stains and soils so that these materials can be washed away more easily.
What are food thickeners made from?
Mostly enzymes or starch molecules which thicken whilst heat or liquid is added.
Why is Ammonia solution added while preparing EDTA solution?
ammonia solution is added during the preparation of EDTA solution to increase the rate of dissolution of its disodium salt.
