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Is absorbance depends on the temp of the room or solvents in spectrophotometric measurement?
Wiki User
∙ 14y ago
The absorbance depends on the concentration of the chemical species to be analyzed (for identic spectrophotometric cells).
Wiki User
∙ 14y ago
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Why do you use the wavelength with the maximum absorbance in spectroscopy?
Short answer:Using the maximum wavelength gives us the best results. This is because at the peak absorbance, the absobance strength of light will be at the highest and rate of change in absorbance with wavelength will be the smallest. Measurements made at the peak absorbance will have the smallest error.Long answer: It really depends on what is the largest source of error. Taking the readings at the peak maximum is best at low absorbance, because it gives the best signal-to-noise ratio, which improves the precision of measurement. If the dominant source of noise is photon noise, the precision of absorbance measurement is theoretically best when the absorbance is near 1.0. So if the peak absorbance is below 1.0, then using the peak wavelength is best, but if the peak absorbance is well above 1.0, you might be better off using another wavelength where the absorbance is closer to 1. Another issue is calibration curve non-linearity, which can result in curve-fitting errors. The non-linearity caused by polychromatic light is minimized if you take readings at either a peak maximum or a minimum, because the absorbance change with wavelength is the smallest at those wavelengths. On the other hand, using the maximum increases the calibration curve non-linearity caused by stray light. Very high absorbances cause two problems: the precision of measurement is poor because the transmitted intensity is so low, and the calibration curve linearity is poor due to stray light. The effect of stray light can be reduced by taking the readings at awavelength where the absorbance is lower or by using a non-linear calibration curve fitting technique. Finally, if spectral interferences are a problem, the best measurement wavelength may be the one that minimizes the relative contribution of spectral interferences (which may or may not be the peak maximum). In any case, don't forget: whatever wavelength you use, you have to use the exact same wavelength for all the standards and samples. See http://terpconnect.umd.edu/~toh/models/BeersLaw.htmlTom O'HaverProfessor Emeritus
What solvents will remove the ink from a permanent marker?
i believe hand sanitzer does.... it depends on the surface. :)
What is the liquid measurement of a tear?
It depends on the tear size.
What is the unit of measurement for matter?
It depends on what you want to measure related to matter.
When something is hypotonic does it move fast?
Hypo and hypertonic have nothing to do with moving fast. They have to do with concentrations of solutes in solvents. The movement is probably molecules and that depends on temperature.
Why do you use the wavelength with the maximum absorbance in spectroscopy?
Short answer:Using the maximum wavelength gives us the best results. This is because at the peak absorbance, the absobance strength of light will be at the highest and rate of change in absorbance with wavelength will be the smallest. Measurements made at the peak absorbance will have the smallest error.Long answer: It really depends on what is the largest source of error. Taking the readings at the peak maximum is best at low absorbance, because it gives the best signal-to-noise ratio, which improves the precision of measurement. If the dominant source of noise is photon noise, the precision of absorbance measurement is theoretically best when the absorbance is near 1.0. So if the peak absorbance is below 1.0, then using the peak wavelength is best, but if the peak absorbance is well above 1.0, you might be better off using another wavelength where the absorbance is closer to 1. Another issue is calibration curve non-linearity, which can result in curve-fitting errors. The non-linearity caused by polychromatic light is minimized if you take readings at either a peak maximum or a minimum, because the absorbance change with wavelength is the smallest at those wavelengths. On the other hand, using the maximum increases the calibration curve non-linearity caused by stray light. Very high absorbances cause two problems: the precision of measurement is poor because the transmitted intensity is so low, and the calibration curve linearity is poor due to stray light. The effect of stray light can be reduced by taking the readings at awavelength where the absorbance is lower or by using a non-linear calibration curve fitting technique. Finally, if spectral interferences are a problem, the best measurement wavelength may be the one that minimizes the relative contribution of spectral interferences (which may or may not be the peak maximum). In any case, don't forget: whatever wavelength you use, you have to use the exact same wavelength for all the standards and samples. See http://terpconnect.umd.edu/~toh/models/BeersLaw.htmlTom O'HaverProfessor Emeritus
Which solvents dissolve Lacquer?
i do not know sorry
What is rf value of eugenol?
The Rf value depends on the type of plate used and solvents.
What solvents will remove the ink from a permanent marker?
i believe hand sanitzer does.... it depends on the surface. :)
What is the molar absorption of copper-II sulfate pentahydrate whose absorbance is 0.17 at 650nm?
Depends on the length the light traveled through the solution and the solution concentration. molar absorption = absorbance/(length x concentration) length is typically in cm and concentration is typically in mol/L
How do you dissolve plastics?
With solvents. The kind of solvent depends on the "plastic" involved. Polystyrene dissolves in benzene or toluene Polyurethane (not the thermoplastic which is insoluble in most solvents) can dissolve in acetone. Polyethylene is resistant to most organic solvents. Styrofoam will dissolve in the isopropanol solvents used in some glues. This can be helpful or hindering when working with arts and crafts.
Do hydrogen chloride is soluble or insoluble in organic solvents?
Depends on solvent properties: Polar organic solvents can solve high amounts of HCl ( like Ethanol, Methanol, THF, Dioxan) In unpolar solvents HCl have low solubility ( like Benzene, Chloroform, Hexane)
How do colligative properties relate to chemistry?
Colligative properties depends only on the concentration of solutes in solvents.
How do you remove construction adhesives?
Depends what you are removing them from and why. Many can be sanded off, some can be removed with solvents.
How do you convert with measurement to degrees?
The answer depends on what measurement the conversion concerns. There is no way that you can convert a measurement of mass to degrees, for example.
How do you find the missing area measurement of a triangle?
depends on the needed measurement and type of triangle.
Why is colligative properties a central part of chemistry?
Colligative properties depends only on the concentration of solutes in solvents.
