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No, it is not valid. Results need to be read within 20 seconds because oxygen in the area quickly distorts the results in an experiment of this kind.

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Q: Is it a valid test if an organism that possesses cytochrome c oxidase and the reagent begins to turn blue at 45 seconds?
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Give explanation why the Oxidase test identifies the presence of cytochrome c oxidase and not the other electron carriers?

An oxidase test relies on a reagent that will change colors when it is oxidized. The reagent used in the test only reacts to cytochrome c oxidase by acting in place of oxygen to receive electrons from the cytochrome.


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