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Is it true that If you cut two sources of DNA with the same restriction enzyme you can then hook them together to form recombinant DNA.?
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To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
Fasle.
What are restriction enzymes Explain the significance of these enzymes in recombinant DNA technology.?
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences known asrestriction sites....................refer in this website en.wikipedia.org/wiki/Restriction_enzyme
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
Why restriction enzyme is also called endonuclease enzyme?
Because these enzymes cut the DNA molecule at a particular site. But like scissors these are useful tools in genetic engineering or recombinant DNA technology.
What is a recombinant enzyme?
Enzymes derived from recombinant DNA technology as opposed to naturally occurring enzymes
To produce a recombinant plasmid and the foreign DNA are cut with a different restriction enzyme?
Fasle.
What is the substance required to cleave the vector DNA during recombinant DNA technology?
A restriction enzyme, also called a restriction endonuclease, is needed to cleave vector DNA when using recombinant DNA technology.
What seals the sticky ends of restriction fragments to make recombinant DNA?
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
What is a sticky end?
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
Which pair of enzymes is necessary to make recombinant DNA?
Actually the answer would be Restriction enzyme and DNA ligase.
What are restriction enzymes Explain the significance of these enzymes in recombinant DNA technology.?
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences known asrestriction sites....................refer in this website en.wikipedia.org/wiki/Restriction_enzyme
How is rcombinant DNA made?
Recombinant DNA is made by combining DNA from different sources. The most common way is to use a plasmid (a circular piece of prokaryote DNA), cut it with a restriction enzyme and then mix it with your target gene which has also been cut with the same enzymes so the pieces fit together like a jigsaw puzzle.
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
Why restriction enzyme is also called endonuclease enzyme?
Because these enzymes cut the DNA molecule at a particular site. But like scissors these are useful tools in genetic engineering or recombinant DNA technology.
Why ligation can be considered the reverse of the restriction enzyme process?
Restriction enzymes cuts out a specific short nucleotide sequence while as the process of ligation, DNA ligase joins them together. So ligase can be considered the reverse of the restriction enzyme process as it joins DNA fragments together instead of cutting them out.
What is a recombinant enzyme?
Enzymes derived from recombinant DNA technology as opposed to naturally occurring enzymes
