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competence is the phenomenon exhibited by the cell during the particular stage of cell cycle by bacteria....it is log phase. During this stage the cell can accept any foreign DNA to enter in to it...so cell are grown upto the log phase or mid log phase and then transformed with the plasmid DNA
What else can I help you with?
What is the action of cacl2 to make a bacterial cell competent?
This is because the Ca ions being positively charged attack both the negatively charged DNA and also the lipopolysaccharide membrane. Thus, the DNA can then pass through the cell on subsequent heat shock treatment.
How does the sodium potassium work?
The sodium-potassium pump is a transportation mechanism in cell membranes that maintains the cell's resting membrane potential by moving sodium out of the cell and potassium into the cell. This process helps regulate cell volume, signaling, and nerve conduction. It is an essential mechanism for proper cellular function and overall physiological balance.
Why you add chloroform in phage stock preparation?
Chloroform is used in phage stock preparation to lyse bacterial cell membranes, releasing phages into solution. It disrupts the lipid bilayer of the cell membrane, helping to release phages from the infected bacteria. This step is crucial for phage extraction and purification.
Can a substance get stuck in a cell?
Yes, certain substances can get trapped inside a cell if they are too large to pass through the cell membrane or if there is a transport mechanism that is not functioning properly. These trapped substances can interfere with normal cellular processes and potentially lead to cell dysfunction or death.
What is the mechanism in which ATP is used to move sodium out of the cell and potassium into the cell restores the resting membrane voltage and intracellular ionic concentrations?
This process is known as the sodium-potassium pump, which uses energy from ATP hydrolysis to pump 3 sodium ions out of the cell and 2 potassium ions into the cell against their concentration gradients. This helps to maintain the resting membrane potential and intracellular ionic concentrations essential for proper cell function.
What is the action of cacl2 to make a bacterial cell competent?
This is because the Ca ions being positively charged attack both the negatively charged DNA and also the lipopolysaccharide membrane. Thus, the DNA can then pass through the cell on subsequent heat shock treatment.
How do you send plasmids to cell?
Herat shock and treating the cells with CaCl2
Why use glycerol in competent cell preparation?
Glycerol is added to competent cell preparations as a cryoprotectant to prevent cell damage and increase cell viability during the freezing and storage process. It helps maintain cell membrane integrity and reduce ice crystal formation, which can damage the cells. Glycerol also aids in the efficient uptake of DNA during transformation by reducing membrane rigidity and enhancing permeability.
How molecular mechanism of bacterial transformation is carried out?
Basically, transformation refers to the uptake and incorporation of naked genetic material by a competent cell. Most bacterial cells are not naturally competent(able to take up naked genetic material). The kinds that are use a type 4 pilli to get the genetic material into the cell. This process is very rare in nature, since it requires naked genetic material to be present, from a lysed cell, as well as a competent cell to take up the genetic material. When the DNA/RNA is taken up, it is often degraded by restriction enzymes. If it is not degraded it can exist as extra chromosomal DNA, much like a plasmid, or it can be incorporated into the cells chromosome. Scientists use heat, pH, or osmotic shock in lab to make cells artificially competent.
What is mechanism of electrochemical cell?
I don't
What does transformation and competent and plasmid mean?
transformation is to introduce a foreign DNA segment to a competent cell. competent cell means a cell that have ability to take a foreign DNA. plasmid is genetic element that one can integrate a DNA segment. it has its promoter, resistance gene, multiple cloning site...
Cellular transport mechanism that depends upon transporters or carrier enzymes in the cell membrane?
Protein the the cell that moves mechanism. This cell also carries enzymes in the membrane.
Cellular transport mechanism that depends upon transporters or carrier enzymes in the cell membrane is?
Protein is the cell that moves mechanism the depends on movement. This cell carries enzymes in the membrane.
What phase does preparation for cell division occur?
during interphase the cells prepare for the divsion and the DNA replicates and chromosomes form
How is mechanism important to cell survival?
rrr
What is connecting mechanism of nervous system?
connecting mechanism is the part of female that reproduce egg cell
The process of a cell's nucleus replicating and dividing in preparation for the division of the cell is called?
mitosis
