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This is because the Ca ions being positively charged attack both the negatively charged DNA and also the lipopolysaccharide membrane. Thus, the DNA can then pass through the cell on subsequent heat shock treatment.

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Mechanism of cacl2 in competent cell preparation?

competence is the phenomenon exhibited by the cell during the particular stage of cell cycle by bacteria....it is log phase. During this stage the cell can accept any foreign DNA to enter in to it...so cell are grown upto the log phase or mid log phase and then transformed with the plasmid DNA


What is the structure of aminoglycosides and how does it contribute to their mechanism of action?

Aminoglycosides are a type of antibiotic with a unique structure that includes multiple amino sugars linked together. This structure allows them to bind to bacterial ribosomes and disrupt protein synthesis, ultimately leading to bacterial cell death. This mechanism of action makes aminoglycosides effective against a wide range of bacterial infections.


What is the method of action for carbenicillin?

Carbenicillin is a beta-lactam antibiotic that inhibits bacterial cell wall synthesis by interfering with the enzymes responsible for cross-linking peptidoglycan chains in the cell wall. This leads to cell wall weakening and eventual lysis of the bacteria.


What happens when a bacterial cell is placed in a solution containing 5 percent NaCl?

When placed in a 5% NaCl solution, a bacterial cell will likely experience water loss through osmosis due to the high salt concentration outside the cell. This will cause the cell to shrink and may lead to dehydration and cell death if the salt concentration is too high.


What happens when a bacterial cell is placed in 10 percent Nacl with penicillin?

When a bacterial cell is placed in a 10% NaCl solution with penicillin, the high salt concentration will cause water to leave the cell, leading to dehydration and shrinkage of the cell. The penicillin will inhibit the synthesis of the bacterial cell wall, making it more susceptible to the osmotic stress induced by the high salt concentration, ultimately leading to cell death.

Related Questions

Mechanism of cacl2 in competent cell preparation?

competence is the phenomenon exhibited by the cell during the particular stage of cell cycle by bacteria....it is log phase. During this stage the cell can accept any foreign DNA to enter in to it...so cell are grown upto the log phase or mid log phase and then transformed with the plasmid DNA


How molecular mechanism of bacterial transformation is carried out?

Basically, transformation refers to the uptake and incorporation of naked genetic material by a competent cell. Most bacterial cells are not naturally competent(able to take up naked genetic material). The kinds that are use a type 4 pilli to get the genetic material into the cell. This process is very rare in nature, since it requires naked genetic material to be present, from a lysed cell, as well as a competent cell to take up the genetic material. When the DNA/RNA is taken up, it is often degraded by restriction enzymes. If it is not degraded it can exist as extra chromosomal DNA, much like a plasmid, or it can be incorporated into the cells chromosome. Scientists use heat, pH, or osmotic shock in lab to make cells artificially competent.


Does a Bacterial cell have only a cell wall?

No, bacterial cell also have phospholipid bilayers.


Is bacterial cell a endo spore?

yes a bacterial cell is an endo spore


Does a bacterial cell have a cell membrane?

Of course they are found in bacterial cells.Every living cell has a plasma membrane.


Which is part is a part of a bacterial cell?

The cell wall is a part of a bacterial cell that provides structure and support.


What does the bacterial cell reproduce that the human gene codes for?

the bacterial cell reproduces the bacterial chromosome that the human gene codes for.


How do you send plasmids to cell?

Herat shock and treating the cells with CaCl2


How do penicillins kill bacteria and what is the mechanism behind their antibacterial action?

Penicillins kill bacteria by disrupting the cell wall synthesis process. They work by inhibiting the enzyme transpeptidase, which is responsible for cross-linking the peptidoglycan chains in the bacterial cell wall. This weakens the cell wall, leading to cell lysis and ultimately bacterial death.


How is bacterial cell different from onion cell?

Bacterial cells are prokaryotic and the cells of an onion are eukaryotic.


What is meant by term competent when applied to bacteria being prepared for transformation?

Being competent refers to the ability of bacteria to uptake foreign DNA. In the context of transformation, bacteria are made competent through the use of special techniques that temporarily disrupt their cell walls, allowing foreign DNA to enter the cells. This process is essential for the successful transfer of new genetic material into bacterial cells.


Does bacterial cell have centrioles?

No because bacterial cells are prokaryotic