How do colonies on the surface of a pour plate differ from those suspended in the agar?
The colonies on the surface of a pour plate are obligate aerobes, they need oxygen to grow. The colonies suspended in the agar of the pour plate is facultative anaerobe, where they can live with or without oxygen.
They have a fuzzy appearance due to the production of spores.
it is used to count the colonies
Agar plates have more surface room so colonies can be seen, and so can its morphology.
A blood agar plate is a nutrient agar plate that has blood added to it before pouring the plates.
Because of their ability to grow on specific environment & the amount of oxygen needed...(e.g. anaerobes, aerotolerants will grow in agar).
To separate out the cells so that they produce individual colonies.
whitw to whitish gray
The following are some advantages of an agar plate verses a slant tube: 1. Surface area- An agar plate has a much larger surface area: a. Easier to isolate individual colonies using the streak-plate method. i. Evaluate the colony shape, margin and elevation. b. Can grow a larger number of cells. 2. Growth- An agar plate allows you to quantify the number of colonies on an agar plate, provided it is within the 30-300 range. Whereas the slant tube cannot quantify growth but only describes growth as none, slight, moderate, or large.
On the base of the agar plate.
to inoculate an agar plate you would place bacteria on the agar to grow
The advantage of the agar plate over the slant is in growing an isolate the bacteria specimens for samples. The slant tube is then used to maintain and grow those isolated colonies.
Function of the agar plate
Agar plate is for isolation of bacteria and fungi. A solid surface makes it easy for bacteria to grow into single colonies. Agar slant is for preserving cultures of bacteria. With screw cap tubes, the agar does not dry up easily. The tubes take very small storage space.
i believe henrici slide
Because the colonies will run together.
Streptococcus pneuomoniae When this organism is tested on a blood agar plate it's colonies will be surrounded by a greenish discoloration due to alpha-hemolysis.
If bateria growson an agar plate it means that the thing that touched the LB Agar or what you swabed was contaminated.
If you are using a plain agar plate, you will have to guess. It all depends on if you are looking for a bacteria or a fungi.
MacConkey Agar plate
condensation from moisture will cause colonies in the pour plate method to not isolate properly