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When using the phenol red carbohydrate broth fermentation test at what pH is the pH indicator yellow?
The pH indicator phenol red turns yellow in the fermentation test when the pH drops below 6.8, indicating acid production from fermentation.
What culture media can be used to test the carbohydrate fermentation?
To test carbohydrate fermentation, common culture media include phenol red broth and MacConkey agar. Phenol red broth contains a specific carbohydrate, and the color change indicates fermentation. MacConkey agar is selective for Gram-negative bacteria and differentiates lactose fermenters, which produce acid, leading to a color change in the medium. Both media are effective for assessing the fermentation capabilities of various microorganisms.
Explain the purpose of the phenol red in the medium?
To determine if a bacterium can ferment a particular carbohydrate and determine the end products of that fermentation
Is a poly phenol a carbohydrate?
NO. They are organic compounds with a hydroxyl group(OH) attached to it.
What substance makes MSA differential?
Mannitol salt agar (MSA) is differential due to the presence of mannitol and phenol red. Mannitol serves as a fermentable carbohydrate, allowing certain bacteria, particularly Staphylococcus species, to ferment it. The fermentation of mannitol produces acid, which lowers the pH and causes the phenol red indicator to change color, turning the agar from red to yellow. This color change differentiates mannitol fermenters from non-fermenters.
What would an organism use for energy in test tube during fermentation and what color would the indicator be.?
Sugar is the energy source, the definition of fermentation is: The anaerobic conversion of sugar to carbon dioxide and alcohol by yeast. As to the color of the indicator it would depend on what you were using to test and what you were testing for (sugar, Co2, alcohol).
What is the purpose of phenol red or bromcresol purple in fermentation tube?
Phenol red or bromcresol purple are pH indicators used in fermentation tubes to detect changes in pH. When bacteria ferment carbohydrates, they produce acidic by-products that cause the indicator to change color based on the pH of the medium, allowing for pH changes to be visualized. This provides a quick way to determine if fermentation has occurred based on the color change observed.
What is molisch?
Molisch's Test refers to a chemical test used to test for carbohydrate existence. This is done using carbohydrate dehydration with hydrochloric or sulfuric acid to create aldehydes. These condense a couple phenol molecules to produce purple or red compounds.
What are the results for Pseudomonas aeruginosa in phenol red broth?
Negative for both. Pseudomonas does not ferment sugars.
Is a phenol considered to be an alcohol?
Yes, phenol is considered to be an alcohol because it has a hydroxyl group (-OH) attached to an aromatic ring.
Why phenol does not dissolve in water when excess?
Phenol is slightly soluble in water due to hydrogen bonding between the hydroxyl group of phenol and water molecules. However, when excess phenol is added, it disrupts the hydrogen bonding network of water molecules, making it less soluble. This is because phenol-phenol interactions become stronger than phenol-water interactions, leading to precipitation.
Drinking phenol a person can die immediately?
Drinking phenol can result to immediate death because it is corrosive and may burn the heart and the kidneys.
