It sequester carbohydrates in the solution
CTAB is a detergent used to denature proteins from samples. Once the protens have been denatures, the isolation of DNA from the non required waste materials can begin.
CTAB is a surfactant used in the isolation of DNA from tissues containing high amounts of polysaccharides. Under the high-salt conditions of this protocol, CTAB binds the polysaccharides removing them from the solution. When combined with Arabidopsis, this procedure yields pure DNA.
The CTAB extraction procedure is from Rogers and Bendich (1986). The magic bullet is supposed to be the separation of polysaccharides from nucleic acids by the use of CTAB. The technique capitalizes on the previous observations that nucleic acids can be selectively precipitated with CTAB. RNA and DNA are soluble in CTAB and 0.7 M NaCl but precipitate when the salt is reduced below 0.4 M. However, many polysaccharides are insoluble over this salt range and are thus not solubilized. CTAB is NOT used to lyse membranes in this procedure.
it act as as a cationic detergent for the isolation dna from the given sample
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
phenol,chloroform,isoamyl alcohol,ethanol,CTAB reagent,Na acetate etc.. but nowadays, they use kits for any kind of DNA isolation, which makes their job easier.
Hello, Its very simple, u can go for the CTAB method , with bit modification, 1) Ensure the youngest possible leaves, very tender enough to crush. 2) Crush the leaf sample in liquid nitrogen. 3) add quickly CTAB buffer to the crushed isolate immediately. 4) the follow Rapid method of isolation.
It solubilizes lipids and a lot of proteins to remove them from the DNA.
the role seveg in plant DNA extractions is to remove chlorophyll and similar pigments
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
none
for cell lysis