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What is the function of restriction enzymes in the process of DNA recombination?
Wiki User
∙ 12y ago
First, a specific enzyme is needed to cut the DNA from the donor genes at a specific site. This enzyme is called a restriction enzyme.The enzyme is used to cut out a piece of DNA that contains one or more desired genes from the donor's DNA.
Next, a vector is needed to receive the donor DNA. Most frequently, a naturally occurring circular piece of bacterial DNA, called a plasmid, is used for this purpose.
Finally, an enzyme is used to "stitch" the donor DNA into the plasmid vector. This enzyme is called ligase, and it creates permanent bonds between the donor DNA and the plasmid DNA. The result is that the donor DNA is incorporated into the bacterial plasmid, forming the recombinant DNA (rDNA)
Wiki User
∙ 10y ago
Wiki User
∙ 13y agoThe process of insertion of a foreign gene in to a vector DNA is known as Recombinant DNA technology.
The enzyme restriction or better known as restriction endonuclease are unique enzyme which can cut or nick in middle of the DNA.so that a foreign DNA can be inserted .and both the vector and foreign DNA can further be joined by enzyme DNA ligases.
Restriction enzyme break the phosphodiester bond whereas ligase create the same bond between two nucleotide.
Wiki User
∙ 12y agocut DNa into smaller segments
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What is meant by the term restriction digest?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
What does a geneticist use to cut DNA at specific base sequences?
Restriction enzymes, also known as restriction endonucleases, are used to cut DNA into smaller fragments. Restriction enzymes are found in bacteria, where they act like molecular scissors by cutting up DNA from invading viruses or bacteriophages. Each restriction enzyme recognizes a specific nucleotide sequence and cuts the DNA at that site. This process makes restriction enzymes extremely useful in biotechnology where they are used in procedures such as DNA cloning, DNA fingerprinting, and genetic engineering. There are hundreds of known restriction enzymes, and each one was named for the bacteria from which it was isolated. For example, EcoRI was isolated from Escherichia coli and HaeIII from Haemophilus aegyptius.
After DNA is cut with a restriction enzymes how is the mixture of DNA fragments sorted?
Through the process of gel electrophoresis.
What process is used to cut DNA into fragments?
DNA can be fragmented using restriction endonucleases or restriction enzymes. Restriction enzymes identify specific sequences within the DNA and cause cleavage generating fragments. When this digested DNA is allowed to run in gel electrophoresis fragments get separated according to their mass. When visualized under UV transilluminator, fragmented DNA can be observed as fluorescing bands.
What are metabolic enzymes?
Metabolic enzymes are acidic proteins that exist in the mitochondria in every cell. Their function is to process ingested proteins and sugars into energy which your body will use to function or replicate cells.
What is meant by the term restriction?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
What is meant by the term restriction digest?
Its the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's).
What does a geneticist use to cut DNA at specific base sequences?
Restriction enzymes, also known as restriction endonucleases, are used to cut DNA into smaller fragments. Restriction enzymes are found in bacteria, where they act like molecular scissors by cutting up DNA from invading viruses or bacteriophages. Each restriction enzyme recognizes a specific nucleotide sequence and cuts the DNA at that site. This process makes restriction enzymes extremely useful in biotechnology where they are used in procedures such as DNA cloning, DNA fingerprinting, and genetic engineering. There are hundreds of known restriction enzymes, and each one was named for the bacteria from which it was isolated. For example, EcoRI was isolated from Escherichia coli and HaeIII from Haemophilus aegyptius.
After DNA is cut with a restriction enzymes how is the mixture of DNA fragments sorted?
Through the process of gel electrophoresis.
Why ligation can be considered the reverse of the restriction enzyme process?
Restriction enzymes cuts out a specific short nucleotide sequence while as the process of ligation, DNA ligase joins them together. So ligase can be considered the reverse of the restriction enzyme process as it joins DNA fragments together instead of cutting them out.
What is the function of the intestinal gland?
Its function is to complete the process begun by the pancreatic juice.
What process is used to cut DNA into fragments?
DNA can be fragmented using restriction endonucleases or restriction enzymes. Restriction enzymes identify specific sequences within the DNA and cause cleavage generating fragments. When this digested DNA is allowed to run in gel electrophoresis fragments get separated according to their mass. When visualized under UV transilluminator, fragmented DNA can be observed as fluorescing bands.
What are metabolic enzymes?
Metabolic enzymes are acidic proteins that exist in the mitochondria in every cell. Their function is to process ingested proteins and sugars into energy which your body will use to function or replicate cells.
Recombination occurs during which process?
Meiosis.
Why are restriction enzymes necessary in the DNA fingerprinting process?
because DNA is the process of getting heriderity informationAns2:Restriction enzymes clip the DNA strand and create short fragments that can be processed. If you clip the strand at a known combination, you will know that every resulting fragment ends with that combination. Knowing the lengths of the fragments allows you to identify where that combination would be located on the complete strand.
If your body temperature is low how will the enzymes work?
Enzymes require certain temperatures to function. They become denatured if the temperature is too high or too low. If an enzyme is denatured, it can no longer function. Therefore, if your body temperature is too low, the enzymes will become denatured and cease to function. The process of denaturation is on a continuum, however. If the temperature is slightly lower than normal, but not too low, the enzymes will still function, but at a lower rate.
What is the process by which genotype becomes expressed as phenotype?
The process by which genotype becomes expressed as phenotype is called recombination. Recombination usually occurs naturally and during meiosis.
