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It is around 4000-6000 plates for a 2 meter column.
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The number of theoretical plates in a chromatography column is a measure of how "long" the column is - how well it separates. A "short" column will only separate large or heavy molecules, and the medium and light stuff is still mixed together in the last band. A "long" column will separate the little stuff better because there are more theorectical plates. Picture a stack of sieves with smaller and smaller holes as the column gets "longer" and you've got the idea. This "length" has virtually nothing to do with the physical length of the separating column. It is a function of the packing materials and solvents used during a separation.
In liquid chromatography the 'theoretical plates' number is a measure of the resolution between the peaks of different eluting substances. The higher the plate value the greater the separation. This is particular important as the load reaches the maximum the column is designed for.
Answer:The factors which affect the chromatography are:TemperaturePressureFlow rate of mobile phaseSample preparation
TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase. GLC. The mobile phase ia s gas, the stationary phase is a liquid on a solid support. same concept as TLC. useful for seperating gases by their affinities to the stationary phase...the mobility can then be compared to known compounds for possible identification.
In the context of distillation and chromatography, the term "16" often refers to the empirical formula used to estimate the number of theoretical plates (N) in a column or separation process. This formula is derived from the relationship between the height of a theoretical plate (H) and the overall column height (L), expressed as ( N = \frac{L}{H} ). The number 16 is commonly associated with the simplified relationship N = (16)(\frac{L}{H}), indicating that the efficiency of a separation process can be linked to the design and operational parameters of the column.
The Different Types of Chromatography There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography. Liquid Chromatography is used in the world to test water samples to look for pollution in lakes and rivers. It is used to analyze metal ions and organic compounds in solutions. Liquid chromatography uses liquids which may incorporate hydrophilic, insoluble molecules. Gas Chromatography is used in airports to detect bombs and is used is forensics in many different ways. It is used to analyze fibers on a persons body and also analyze blood found at a crime scene. In gas chromatography helium is used to move a gaseous mixture through a column of absorbent material. Thin-layer Chromatography uses an absorbent material on flat glass or plastic plates. This is a simple and rapid method to check the purity of an organic compound. It is used to detect pesticide or insecticide residues in food. Thin-layer chromatography is also used in forensics to analyze the dye composition of fibers. Paper Chromatography is one of the most common types of chromatography. It uses a strip of paper as the stationary phase. Capillary action is used to pull the solvents up through the paper and separate the solutes.
It is the efficiency of the column. The larger the number, the more theoretical plates the column possesses; a typical well-packed column with a 5-micrometer particle size porous packing in a 15cm x 46 mm column should provide10,000-20,000 plates. sorry. I forgot to put a point in there. It should be 4.5 mm, not 45
The Different Types of Chromatography There are four main types of chromatography. These are Liquid Chromatography, Gas Chromatography, Thin-Layer Chromatography and Paper Chromatography. Liquid Chromatography is used in the world to test water samples to look for pollution in lakes and rivers. It is used to analyze metal ions and organic compounds in solutions. Liquid chromatography uses liquids which may incorporate hydrophilic, insoluble molecules. Gas Chromatography is used in airports to detect bombs and is used is forensics in many different ways. It is used to analyze fibers on a persons body and also analyze blood found at a crime scene. In gas chromatography helium is used to move a gaseous mixture through a column of absorbent material. Thin-layer Chromatography uses an absorbent material on flat glass or plastic plates. This is a simple and rapid method to check the purity of an organic compound. It is used to detect pesticide or insecticide residues in food. Thin-layer chromatography is also used in forensics to analyze the dye composition of fibers. Paper Chromatography is one of the most common types of chromatography. It uses a strip of paper as the stationary phase. Capillary action is used to pull the solvents up through the paper and separate the solutes.
A Lally Lock column is a concrete filled steel column that does not need to have the cap or base plates welded. The top end of the column is fitted with an embedded fastening unit that will accept a 1/2" screw to fasten the cap plate onto the column. The bottom end it just concrete and can be cut with a pipe cutter. Base plates are fastened with concrete screws.
A Lally Lock column is a concrete filled steel column that does not need to have the cap or base plates welded. The top end of the column is fitted with an embedded fastening unit that will accept a 1/2" screw to fasten the cap plate onto the column. The bottom end it just concrete and can be cut with a pipe cutter. Base plates are fastened with concrete screws.