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How can you calculate absorbance of unknown from linear equation?
To calculate the absorbance of an unknown sample using a linear equation, you first need to establish a calibration curve by plotting the absorbance values of known standards against their concentrations. The resulting linear equation, typically in the form (y = mx + b), relates absorbance (y) to concentration (x), where (m) is the slope and (b) is the y-intercept. By measuring the absorbance of the unknown sample and substituting this value into the linear equation, you can solve for the concentration of the unknown sample. This allows you to determine the absorbance based on its concentration derived from the calibration curve.
Why is it important to measure the absorbance of the solution with the lowest concentration first?
It is important to measure the absorbance of the solution with the lowest concentration first because it helps establish a baseline for comparison. This allows for accurate determination of the concentration of other solutions by ensuring that the measurements are within the detection range of the instrument.
How do you find the concentration of potato cells immersed in salt solutions?
You would have to use a spectrophotometer to measure the absorbance of your unknown solution. But first, you need to make several solutions with known concentrations. Measure the absorbance of the known concentrations and plot them on an X and Y axis where X equals concentration and Y equals absorbance. Do a best-fit line for your data. Measure the absorbance of your unknown solution. Find this value on your Y-axis and find out where this value intersects with your line of best fit. The X value at the intersection is your concentration of potato cells. By the way, make sure you use the same wavelength throughout the experiment.
What is Delaware's state nickname?
* First State * Diamond State * Blue Hen State * First State * Diamond State * Blue Hen State
What is Delaware's nickname?
the dimond state, the first state, and the blue hen state
Nevada was the first state to what?
First state to nothing. Da! First state to nothing. Da!
What was the first state to have a state constitution?
Connecticut was the first state to have a state constitution.
Which was the first entered into the united state's?
Delaware was the first state to enter the U.S. of A.
What state is Nickname of the first state?
Delaware has the nickname of The First State because it was the first to ratify the Constitution
Griess manual METHOD for nitric oxide?
The Griess assay is a simple colorimetric method used to quantify the levels of nitric oxide (NO) in a sample. In this method, nitrate and nitrite are first converted to nitrite by a nitrate reductase enzyme. The nitrite is then reacted with a Griess reagent to form a purple compound whose absorbance is measured at 540 nm. This absorbance is directly proportional to the concentration of NO in the sample.
What state was the first state to vote in the primaries in 2008?
Iowa was the first state.
Was it the first or second or third state?
It was the first state.
