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The activity of which enzyme decreases in both acidic and basic envioroment?
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What can you do to regain the activity of the enzyme?
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
What are the effects of freezing on enzyme activity?
When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.
What is the effect of cold temperatures on enzyme activity?
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
What blocks enzyme activity by binding to allosteric site of an enzyme causing the enzyme's active site to change shape?
An allosteric inhibitor stops enzyme activity by binding to an allosteric site and causing the conformation of the enzyme to change.
What does activity of an enzyme depend on?
The concentration of hydrogen ions in solution affects the enzyme activity. Each enzyme has maximal efficiency under an optimum pH. Since pH is one of the factors for the denaturation of proteins, if an enzyme is submitted to a pH level under which it is denatured there will be no enzymatic activity.
How do you use inhibitor in a sentence?
An enzyme inhibitor is a substance that binds to an enzyme and decreases the enzyme's activity.
How might amino acid change affect the enzyme?
The pKA of enzyme affects its ionization which could alter enzyme activity. For pH < pKa, the value of vmax is constant and that for pH > pKa, vmax decreases; ie. enzyme activity starts to decline.
Why might enzyme's activity decrease at very high temperatures?
Enzymes activity is affected by temperature. At a very high temperature, enzymes became denature that means they lose their original shape, which is important for them to react. Thus, enzyme activity decreases at a very high temperature.
What is the effect of pH upon the relative enzyme activity?
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied
What can you do to regain the activity of the enzyme?
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
What is the effect of hydrochloric acid on the enzyme activity?
Denature enzyme activity
What are the effects of physical activity on enzyme activity?
Physical activity can alter the shape of enzyme which can cause damage or may the enzyme become inactive
What are some factors that may influence enzyme activity?
Factors affecting enzyme activity1: Enzyme concentrationIf the quantity of enzyme is doubled, the enzymatic activity will also be doubled because more enzymes are now available to work. After a certain level of enzyme concentration, there will be no more increase in the enzymatic activity because all the substrate molecules are combined with an enzyme and the rate of reaction will stabilize.2: Substrate concentrationBy increasing substrate concentration, enzymatic activity increases. Increasing the substrate further without increasing the enzyme concentration will not affect the enzymatic activity because all the enzymes are occupied by a substrate molecule.3: pH valueSome enzymes require acidic surroundings, most require a more neutral condition for their activity. Change in the pH can change the enzyme's structure and enzyme become useless.4: TemperatureAn increase in temperature of 10 degree celsius doubles the enzymatic activity. Each enzyme has its own optimum temperature at which its enzymatic activity is maximum. Very high temperatures break the bonds that maintain shape of enzyme. If the enzyme denatures, the substrate can not fit in to the active sites and enzyme become useless.
What are the effects of freezing on enzyme activity?
When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.
What is the Effect of dilution on enzyme activity?
At a high ion concentration, the ion interfere with the bonds between the side groups of the amino acids making up the enzyme (which is a protein). This causes the enzyme to lose its shape, called denaturation. If the enzyme loses its shape, it can no longer accept and react substrate, so the rate of enzyme activity decreases.
What switchs on enzyme activity while what can switch off or reduce enzyme activity?
activators; inhibitors
What is the effect of cold temperatures on enzyme activity?
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
