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What is the function of sulphuric acid in the experiment of enzyme activity?
Wiki User
∙ 11y ago
H2SO4 is used to denature the enzyme and stop the reaction instantly. by adding H2SO4,it will prevent further reaction of the enzyme onto the substrate and the rate of enzyme reaction can be measured in the specific time
Wiki User
∙ 11y ago
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Zinc an essential trace element for most organisms is present in the active site of the enzyme carboxypeptidase The zinc most likely functions as a?
Cofactor necessary for enzyme activity
What happens when iodine reacts with starch that has been digested with amylase?
When iodine is added to an amylaze enzyme activity in an experiment such as a agar plate with wells, it does two things. When tested with starch, the color of the liquid will turn a brownish-yellowish color meaning that enzyme reactions had occured. Also on a well agar plate the idione will repel in a circle around the well. most of the time around 2 cm in diameter.
Why does denaturing by temperature or pH make an enzyme non-functional?
Active site of an enzyme is fixed for particular substrate, or you can understand it better via lock-key theory, in which substrate acts as a lock and active side of the enzyme as a key, and you know well that each lock has a specific key to make it unlock. So if active site of an enzyme is altered due to any reason, it can not be fixed into the its particular substrate, and functioning of an enzyme obliviously requires a substrate.
What is zinc pca?
zinc salt of L-pyrrolidone carboxylic acid. It inhibit the enzyme that catalyses the production of dihydroxytestosterone, a hormone that controls sebaceous gland activity. Zinc is also an antibacterial, and its salts are used to control dandruff.
What enzyme synthesises starch from glucose -1- phosphate?
this enzyme is called phosphorylase.
What is the function of zinc in the active site of the enzyme carboxypeptidase?
cofactor necessary for enzyme activity
In a paper on the catalytic activity of an enzyme, in which section would you find detailed information on how the enzyme was prepared and handled in the experiment?
materials and methods
What is the purpose of the extracellular enzyme experiment?
Extracellular enzyme is an enzyme that performs its role or function outside a cell. The purpose of experimenting extracellular enzyme is to know how can it affect our body when the bacteria secretes.
What is an example of a sentence using the word 'enzyme'?
"During this experiment, we will be looking to see the effects of heat on enxyme activity" "This stain remover contains an enzyme"
In a paper on catalytic activity of an enzyme in which section would you find detailed information on how the enzyme was prepared and handled in the experiment?
Material and method
What is the independent variable between temperature and enzyme activity?
To find out how enzyme concentration affects the activity of the enzyme you must:vary the concentration of the enzyme, by preparing different concentrations (keeping the volume of solution the same)keep the temperature, substrate concentration and pH constantmeasure the activity of the enzyme at each concentrationHow the enzyme activity is measured will depend on the specific enzyme involved.You need to have plenty of substrate (excess substrate) so it doesn't run out during the experiment.In this type of experiment, the enzyme activity is the dependent variable, the temperature, pH and substrate concentration are control variables and the enzyme concentration is the independent variable.
What can you do to regain the activity of the enzyme?
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
What happens when enzyme reaches optimal temperature?
The optimal function of the enzyme is impeded and if the temperature rises too high the enzyme, mostly protein, will degrade and become useless.
What is the effect of hydrochloric acid on the enzyme activity?
Denature enzyme activity
What are the effects of physical activity on enzyme activity?
Physical activity can alter the shape of enzyme which can cause damage or may the enzyme become inactive
What does cooling and heating do to enzyme activity?
Cooling generally slows down enzyme activity by decreasing the kinetic energy of the enzyme molecules, limiting their ability to collide with substrate molecules and facilitating the formation of enzyme-substrate complexes. Heating, on the other hand, initially increases enzyme activity by providing more kinetic energy for enzyme-substrate collisions and enhancing the rate of reaction. However, excessive heat can denature enzymes, causing them to lose their shape and function, ultimately decreasing enzyme activity.
What does the wrong pH do to the shape of an enzyme?
It will affect the shape and lead to reduced activity.
